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PXD024279

PXD024279 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification ubiquitination of EGFR by LC-MS/MS
DescriptionPreviously, analysis of EGF-induced EGFR ubiquitination by mass spectrometry revealed that ubiquitinated lysine residues were located in the TKD of EGFR. However, the specific lysine residues for the CBL-mediated polyubiquitination have not been identified. To investigate the differences in EGFR ubiquitination by ZNRF1 and CBL, we sought to identify the acceptor residues for polyubiquitin chains mediated by these two E3 ubiquitin ligases. We co-transfected HEK293T cells with EGFR and ZNRF1 or CBL, and immunoprecipitated EGFR for liquid chromatography-tandem mass spectrometry analysis.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_15:54:08.260.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD024279
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPang-Hung Hsu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ FT; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-22 01:20:00ID requested
12022-02-16 15:54:09announced
Publication List
Shen CH, Chou CC, Lai TY, Hsu JE, Lin YS, Liu HY, Chen YK, Ho IL, Hsu PH, Chuang TH, Lee CY, Hsu LC, ZNRF1 Mediates Epidermal Growth Factor Receptor Ubiquitination to Control Receptor Lysosomal Trafficking and Degradation. Front Cell Dev Biol, 9():642625(2021) [pubmed]
Keyword List
submitter keyword: EGFR, ubiquitination, ZNRF1, CBL, LC-MS/MS
Contact List
Pang-Hung Hsu
contact affiliationNational Taiwan Ocean University
contact emailphsu@ntou.edu.tw
lab head
Pang-Hung Hsu
contact affiliationNational Taiwan Ocean University
contact emailphsu@ntou.edu.tw
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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