PXD024279

PXD024279 is an original dataset announced via ProteomeXchange.

Title	Identification ubiquitination of EGFR by LC-MS/MS
Description	Previously, analysis of EGF-induced EGFR ubiquitination by mass spectrometry revealed that ubiquitinated lysine residues were located in the TKD of EGFR. However, the specific lysine residues for the CBL-mediated polyubiquitination have not been identified. To investigate the differences in EGFR ubiquitination by ZNRF1 and CBL, we sought to identify the acceptor residues for polyubiquitin chains mediated by these two E3 ubiquitin ligases. We co-transfected HEK293T cells with EGFR and ZNRF1 or CBL, and immunoprecipitated EGFR for liquid chromatography-tandem mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_15:54:08.260.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024279
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pang-Hung Hsu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-22 01:20:00	ID requested
⏵ 1	2022-02-16 15:54:09	announced

Shen CH, Chou CC, Lai TY, Hsu JE, Lin YS, Liu HY, Chen YK, Ho IL, Hsu PH, Chuang TH, Lee CY, Hsu LC, ZNRF1 Mediates Epidermal Growth Factor Receptor Ubiquitination to Control Receptor Lysosomal Trafficking and Degradation. Front Cell Dev Biol, 9():642625(2021) [pubmed]

submitter keyword: EGFR, ubiquitination, ZNRF1, CBL, LC-MS/MS

Pang-Hung Hsu
contact affiliation	National Taiwan Ocean University
contact email	phsu@ntou.edu.tw
lab head
Pang-Hung Hsu
contact affiliation	National Taiwan Ocean University
contact email	phsu@ntou.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024279

PRIDE project URI

• PRIDE
  1. PXD024279
     1. Label: PRIDE project
     2. Name: Identification ubiquitination of EGFR by LC-MS/MS