PXD024276

PXD024276 is an original dataset announced via ProteomeXchange.

Title	Trastuzumab modulates the protein cargo of extracellular vesicles released by ERBB2+ breast cancer cells
Description	Cancers overexpressing the ERBB2 oncogene are aggressive and associated with a poor prognosis. Trastuzumab is a ERBB2 specific recombinant antibody employed for the treatment of these diseases since it blocks ERBB2 signaling causing growth arrest and survival inhibition. While the effects of Trastuzumab on ERBB2 cancer cells are well known, those on the extracellular vesicles released from these cells are scarce. This study focused on ERBB2+ breast cancer cells and aimed to establish what type of EVs they release and whether Trastuzumab affects their morphology and molecular composition. To these aims, we performed immunoelectron microscopy, immunoblot, and high-resolution mass spectrometry analyses on EVs purified by differential centrifugation of culture supernatant. Here we show that EVs released from ERBB2+ breast cancer cells are polymorphic in size and appearance, and that ERBB2 is preferentially associated with large (120 nm) EVs. Moreover, we report that Tz induces the expression of a specific glycosylated 50 kDa isoform of the CD63 tetraspanin and modulates the expression of 51 EVs proteins, including TOP1. As these proteins are functionally associated with organelle organization, cytokinesis, and response to lipids, we suggest that Tz may influence these cellular processes in target cells at distant sites via modified EVs.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:10:39.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Petretto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-22 00:20:18	ID requested
⏵ 1	2021-09-09 13:10:40	announced

Marconi S, Santamaria S, Bartolucci M, Stigliani S, Aiello C, Gagliani MC, Bellese G, Petretto A, Cortese K, Castagnola P, Breast Cancer Cells. Membranes (Basel), 11(3):(2021) [pubmed]

submitter keyword: trastuzumab, HER2, extracellular vesicles, breast cancer, proteomic analysis, electron microscopy, TOP1, CD63.

Andrea Petretto
contact affiliation	Ist. G. Gaslini
contact email	a.petretto@gmail.com
lab head
Andrea Petretto
contact affiliation	Gaslini Children's Hospital
contact email	a.petretto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024276

PRIDE project URI

• PRIDE
  1. PXD024276
     1. Label: PRIDE project
     2. Name: Trastuzumab modulates the protein cargo of extracellular vesicles released by ERBB2+ breast cancer cells