PXD024272

PXD024272 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.
Description	We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each culture condition were subjected to albumin depletion followed by DIA-MS. We further analyzed proteins in the conditioned media of HeLa cells with and without tumor necrosis factor (TNF) stimulation.
HostingRepository	jPOST
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-09-18_03:54:07.559.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	中村　蓮
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxymethyl-L-cysteine; unknown modification
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-21 20:51:11	ID requested
1	2022-02-21 07:00:04	announced
⏵ 2	2022-09-18 03:54:08	announced	2022-09-18: Updated FTP location.

Publication List

Nakamura R, Nakajima D, Sato H, Endo Y, Ohara O, Kawashima Y, A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum. Int J Mol Sci, 22(5):(2021) [pubmed]

Nakamura R, Nakajima D, Sato H, Endo Y, Ohara O, Kawashima Y, A Simple Method for In-Depth Proteome Analysis of Mammalian Cell Culture Conditioned Media Containing Fetal Bovine Serum. Int J Mol Sci, 22(5):(2021) [pubmed]

Keyword List

submitter keyword: Proteome analysis, Secreted Proteins, Albumin removal, conditioned medium, Fetal bovine serum, DIA

submitter keyword: Proteome analysis, Secreted Proteins, Albumin removal, conditioned medium, Fetal bovine serum, DIA

Contact List

Yusuke Kawashima
lab head
中村　蓮
contact affiliation	公益財団法人かずさDNA研究所
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001098/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001098/