PXD024250

PXD024250 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of koala (Phascolarctos cinereus) spermatozoa and prostatic bodies
Description	The aims of this study were to investigate the proteome of koala (Phascolarctos cinereus) spermatozoa and that of the prostatic bodies with which they form intimate contact at the moment of ejaculation. For this purpose, spermatozoa and prostatic bodies were obtained from four adult male koala by electroejaculation and analysed by high performance liquid chromatography coupled to a mass spectrometer. This strategy successfully identified and 744 koala sperm and 1,297 prostatic body proteins, which were subsequently attributed to 482 and 776 unique gene products, respectively. Gene ontology curation of the koala sperm proteome revealed an abundance of proteins mapping to the canonical sirtuin and 14-3-3 signalling pathways. By contrast, protein ubiquitination and unfolding protein response pathways dominated the equivalent analysis of proteins uniquely identified in koala prostatic bodies. In terms of functional annotation, koala sperm proteins commonly mapped to the categories of cellular compromise/inflammatory response, whilst those of the prostatic body revealed an over-representation of molecular chaperone and stress-related proteins associated with cell survival. Cross-species comparisons demonstrated that the koala sperm proteome displays greater conservation with that of eutherians (human; 93%) as opposed to reptile (crocodile; 39%) and avian (rooster; 27%) spermatozoa. Similarly, koala prostatic body proteins also presented a relatively high degree of overlap (70%) with those identified in sub-populations of large human seminal plasma extracellular vesicles. Together, this work contributes our overall understanding of the core sperm proteome and has identified candidate markers that may contribute to the exceptional longevity of koala spermatozoa during ex vivo storage.
HostingRepository	PRIDE
AnnounceDate	2021-08-23
AnnouncementXML	Submission_2021-08-23_00:10:58.030.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024250
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Phascolarctos cinereus; NCBI TaxID: 38626;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 21:59:15	ID requested
⏵ 1	2021-08-23 00:10:58	announced

Skerrett-Byrne DA, Anderson AL, Hulse L, Wass C, Dun MD, Bromfield EG, De Iuliis GN, Pyne M, Nicolson V, Johnston SD, Nixon B, Proteomic analysis of koala (phascolarctos cinereus) spermatozoa and prostatic bodies. Proteomics, 21(19):e2100067(2021) [pubmed]

submitter keyword: Koala, Prostatic bodies, Proteomics, Spermatozoa,

Professor Brett Nixon
contact affiliation	Reproductive Science Group, Priority Research Centre for Reproductive Science School of Environmental and Life Sciences College of Engineering, Science and Environment
contact email	Brett.Nixon@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	The University of Newcastle
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024250
     1. Label: PRIDE project
     2. Name: Proteomic analysis of koala (Phascolarctos cinereus) spermatozoa and prostatic bodies