PXD024247

PXD024247 is an original dataset announced via ProteomeXchange.

Title	Mouse Heart SS31 Targeted Proteomics
Description	The S-glutathionylation and phosphorylation proteomes of mouse hearts were analyzed using shotgun proteomics methods in order to assess the effects of aging and the ability of mitochondrion-targeted drug SS-31 to reverse age-related changes. There was a nearly universal increase in S-gluthationylation of cysteine residues on proteins taken from Old (24 months old at the start of the study) mouse hearts compared to Young (5-6 months old). This shift in proteome oxidation state was almost completely reversed by 8-weeks of SS-31 treatment. Many of the significant changes that occurred were in mitochondrial pathways. Changes in phosphorylation did not show a clear pattern, as there was a mix of enhancement and suppression of phosphorylation with age among different proteins. SS-31 showed some effect at restoring the phosphorylation state of proteins that had increased phosphorylation with age but it had no effect on those that had decreased phosphorylation with age. A subset of phosphorylation sites was also analyzed by parallel reaction monitoring, which revealed that Myot S231 had a change in the proportion of phosphorylated and unphosphorylated proteins and that cMyBP-C S307 had a proportional increase in phosphorylated and unphosphorylated protein with age, but that SS-31 treatment did not affect these changes.
HostingRepository	PanoramaPublic
AnnounceDate	2022-03-16
AnnouncementXML	Submission_2022-03-16_13:51:53.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gennifer Merrihew
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Phospho; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 20:03:42	ID requested
⏵ 1	2022-03-16 13:51:54	announced

Whitson JA, Mart, í, n-P, é, rez M, Zhang T, Gaffrey MJ, Merrihew GE, Huang E, White CC, Kavanagh TJ, Qian WJ, Campbell MD, MacCoss MJ, Marcinek DJ, Vill, é, n J, Rabinovitch PS, Elamipretide (SS-31) treatment attenuates age-associated post-translational modifications of heart proteins. Geroscience, 43(5):2395-2412(2021) [pubmed]

submitter keyword: Aging, PRM mass spectrometry, phosphoproteomics

Michael MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
lab head
Gennifer Merrihew
contact affiliation	University of Washington
contact email	genn@uw.edu
dataset submitter

Panorama Public dataset URI