PXD024244

PXD024244 is an original dataset announced via ProteomeXchange.

Title	Proteomic differences between methicillin resistant Staphylococcus aureus showing synergistic effects with cannabinoids
Description	Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen in both community and health care settings, which causes a wide range of infections. Its resistance to β-lactam antibiotics and methicillin in particular, greatly complicates treatment options and success rate due to the limited number of antibiotics with activity against MRSA. To further the development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. Cannabinoid compounds including cannabidiol (CBD), tetrahydrocannabinol (THC) and cannabinol (CBN) have shown promise as potential antibiotic adjuvants. In the present study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds, and subsequently analysed using metaproteomics to assess the metabolic response. Subjecting MRSA to methicillin made the cells more viable and increased their energy production, as well as upregulation of penicillin-binding protein 2 (PBP2). The cannabinoids all showed antimicrobial activity against MRSA, and inhibited the energy production of the cells as well as PBP2 when used in combination with methicillin. Furthermore, all three cannabinoid compounds inhibited resistance mechanisms in MRSA, resulting in a decrease in the minimum inhibitory concentration (MIC) of methicillin when used in combination.
HostingRepository	PRIDE
AnnounceDate	2022-01-18
AnnouncementXML	Submission_2022-01-18_10:04:58.657.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024244
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Struckmann Poulsen
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 10:12:11	ID requested
⏵ 1	2022-01-18 10:04:59	announced

Dataset with its publication pending

submitter keyword: Antibiotic resistance, MRSA, Methicillin, Cannabinoids, Hormesis, Proteomics

Jeppe Lund Nielsen
contact affiliation	Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220, Aalborg East, Denmark
contact email	jln@bio.aau.dk
lab head
Jan Struckmann Poulsen
contact affiliation	Aalborg University
contact email	jsp@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024244
     1. Label: PRIDE project
     2. Name: Proteomic differences between methicillin resistant Staphylococcus aureus showing synergistic effects with cannabinoids