PXD024232

PXD024232 is an original dataset announced via ProteomeXchange.

Title	Proteome profile of Methicillin-Resistant Staphylococcus aureus Membrane-Derived Vesicles grown in presence of sub-MIC dosage of Vancomycin
Description	Methicillin-resistant Staphylococcus aureus (MRSA) has evolved numerous antimicrobial resistance mechanisms and is identified as a serious public health threat by the World Health Organization and U.S. Centers for Disease Control and Prevention. The glycopeptide vancomycin (VAN) remains a cornerstone of therapy for severe MRSA infections despite increasing reports of therapeutic failure in hospitalized patients with bacteremia or pneumonia. Here we examined the effectiveness of MVs-derived methicillin-resistant SA (MRSA) to counteract vancomycin (VAN). We found that supplementation of a typical MIC assay using bacteriologic and tissue-mimicking media with purified MVs attenuated MRSA susceptibility to VAN but no other examined cell-wall targeting antibiotics. In addition, the purified MVs protected MRSA challenged with sub-therapeutic dosage of VAN against the host’s innate immune system. Additionally, the proteome of MV peptides from VAN exposed MRSA was characterized to determine if protein expression was altered.
HostingRepository	PRIDE
AnnounceDate	2022-02-18
AnnouncementXML	Submission_2022-02-18_09:16:42.343.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack-Ansgar Bruun
SpeciesList	scientific name: Staphylococcus aureus subsp. aureus USA300_TCH1516; NCBI TaxID: 451516;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 01:43:07	ID requested
⏵ 1	2022-02-18 09:16:42	announced

Dataset with its publication pending

submitter keyword: MRSA, MVs, Vancomycin, LC-MSMS

Mona Johannessen
contact affiliation	Research group for Host-Microbe Interaction Department of Medical Biology Faculty of Health Sciences UiT- The Arctic University of Norway 9037 Tromsø NORWAY
contact email	mona.johannessen@uit.no
lab head
Jack-Ansgar Bruun
contact affiliation	University of Tromsø
contact email	jack-ansgar.bruun@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024232

PRIDE project URI

• PRIDE
  1. PXD024232
     1. Label: PRIDE project
     2. Name: Proteome profile of Methicillin-Resistant Staphylococcus aureus Membrane-Derived Vesicles grown in presence of sub-MIC dosage of Vancomycin