PXD024230

PXD024230 is an original dataset announced via ProteomeXchange.

Title	Proteoglycan profiling of human, rat and mouse insulin-secreting cells
Description	Proteoglycans (PGs) are proteins with glycosaminoglycan (GAG) chains, such as chondroitin sulfate (CS) or heparan sulfate (HS), attached to serine residues. We have earlier shown that prohormones can carry CS, thus, constituting a novel class of PGs. The mapping of GAG modifications of proteins in endocrine cells may thus assist us in delineating possible roles of PGs in endocrine cellular physiology. With this aim, we applied a glycoproteomic approach to identify PGs, their GAG chains and their attachment sites in insulin-secreting cells. Glycopeptides carrying GAG chains were enriched from human pancreatic islets, rat (INS-1 832/13) and mouse (MIN6, NIT-1) insulinoma cell lines by ion exchange chromatography, depolymerized with GAG lyases, and analyzed by nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS/MS). We identified CS modifications of chromogranin-A, islet amyloid polypeptide, secretogranin 1 and secretogranin 2, immunoglobulin superfamily member 10, and protein AMBP. Additionally, we identified two HS-modified prohormones (chromogranin-A and secretogranin-1), which was surprising, as prohormones are not typically regarded as HSPGs. For chromogranin-A, the glycosylation site carried either CS or HS, making it a so-called hybrid site. Additional HS sites were found on syndecan-1, syndecan-4, nerurexin-2, protein NDNF, and testican-1. These results demonstrate that several prohormones, and other constituents of the insulin-secreting cells are PGs. Cell-targeted mapping of the GAG glycoproteome forms an important basis for better understanding of endocrine cellular physiology, and the novel CS and HS sites presented here provide important knowledge for future studies.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_15:49:04.035.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Nilsson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 01:39:08	ID requested
⏵ 1	2022-02-16 15:49:07	announced

Nikpour M, Nilsson J, Persson A, Noborn F, Vorontsov E, Larson G, Proteoglycan profiling of human, rat and mouse insulin-secreting cells. Glycobiology, 31(8):916-930(2021) [pubmed]

submitter keyword: Chromogranin-A, glycosaminoglycan, insulin, insulinoma islet cells, proteoglycan

Göran Larson
contact affiliation	Department of Laboratory Medicine, Sahlgrenska academy, University of Gothenburg, Gothenburg, Sweden
contact email	goran.larson@clinchem.gu.se
lab head
Jonas Nilsson
contact affiliation	University of Gothenburg
contact email	jonas.nilsson@clinchem.gu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024230
     1. Label: PRIDE project
     2. Name: Proteoglycan profiling of human, rat and mouse insulin-secreting cells