PXD024227

PXD024227 is an original dataset announced via ProteomeXchange.

Title	USP32 regulates non-proteolytic LAMTOR1 ubiquitination and Ragulator function
Description	Ubiquitination is among the most prevalent post-translational modifications regulating a great number of cellular functions, and defects in such processes contribute to many diseases. Deubiquitinating enzymes (DUBs) are essential to control the precise outcome of ubiquitin signals. The ubiquitin-specific protease 32 (USP32) differs from all other DUBs as it comprises in addition to its catalytic USP domain and the DUSP domain also multiple EF hands and a C-terminal prenylation site. This unique domain architecture offers various features for the regulation of specific signaling processes by USP32. A SILAC-based proteomic analysis of purified lysosomes revealed an increase in lysosomal hydrolases in organelles isolated from USP32 KO cells as compared to WT cells, suggesting that hydrolases are not sorted correctly to their destination in the absence of USP32.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:22.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Bonn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-17 23:17:25	ID requested
1	2022-12-16 12:20:16	announced
⏵ 2	2023-11-14 08:24:23	announced	2023-11-14: Updated project metadata.

Hertel A, Alves LM, Dutz H, Tascher G, Bonn F, Kaulich M, Dikic I, Eimer S, Steinberg F, Bremm A, USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction. Cell Rep, 41(10):111653(2022) [pubmed]

submitter keyword: Lysosomes, Ubiquitin

Anja Bremm
contact affiliation	Institute of Biochemistry II, University Hospital, Goethe University, Frankfurt, Germany
contact email	Bremm@em.uni-frankfurt.de
lab head
Florian Bonn
contact affiliation	University of Frankfurt
contact email	flobonn@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD024227

PRIDE project URI

• PRIDE
  1. PXD024227
     1. Label: PRIDE project
     2. Name: USP32 regulates non-proteolytic LAMTOR1 ubiquitination and Ragulator function