PXD024225

PXD024225 is an original dataset announced via ProteomeXchange.

Title	Role of Epigenetics in Unicellular to Multicellular Transition in Dictyostelium
Description	The evolution of multicellularity is a critical event that remains incompletely understood. We use the social amoeba, Dictyostelium discoideum, one of the rare organisms that exists in both unicellular and multicellular stages, to examine the role of epigenetics in regulating multicellularity. While transitioning to multicellular states, patterns of H3K4 methylation and H3K27 acetylation significantly change. By combining transcriptomics, epigenomics, chromatin accessibility, and syntenic analyses with other unicellular and multicellular organisms, we identify 52 conserved genes, which are specifically accessible and expressed during multicellular states. We validated that four of these genes, including the H3K27 deacetylase hdaD, are necessary and that an SMC-like gene, smcl1, is sufficient for multicellularity. These results highlight the importance of epigenetics in reorganizing chromatin architecture to facilitate the evolution of multicellularity.
HostingRepository	PanoramaPublic
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-15_09:40:48.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Lindsay Pino
SpeciesList	scientific name: Dictyostelium discoideum AX2; NCBI TaxID: 366501;
ModificationList	Methyl; Propionyl; Delta:H(8)C(6)O(2); Acetyl
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-17 19:59:50	ID requested
1	2021-02-19 06:23:11	announced
⏵ 2	2021-06-15 09:40:49	announced	: PubMed link added

Wang SY, Pollina EA, Wang IH, Pino LK, Bushnell HL, Takashima K, Fritsche C, Sabin G, Garcia BA, Greer PL, Greer EL, Role of epigenetics in unicellular to multicellular transition in Dictyostelium. Genome Biol, 22(1):134(2021) [pubmed]

submitter keyword: epigenetics, histone, multicellularity

Lindsay Pino
contact affiliation	University of Pennsylvania
contact email	lindsay.pino@pennmedicine.upenn.edu
lab head
Lindsay Pino
contact affiliation	University of Pennsylvania
contact email	lindsay.pino@gmail.com
dataset submitter

Panorama Public dataset URI