<<< Full experiment listing


PXD024225 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRole of Epigenetics in Unicellular to Multicellular Transition in Dictyostelium
DescriptionThe evolution of multicellularity is a critical event that remains incompletely understood. We use the social amoeba, Dictyostelium discoideum, one of the rare organisms that exists in both unicellular and multicellular stages, to examine the role of epigenetics in regulating multicellularity. While transitioning to multicellular states, patterns of H3K4 methylation and H3K27 acetylation significantly change. By combining transcriptomics, epigenomics, chromatin accessibility, and syntenic analyses with other unicellular and multicellular organisms, we identify 52 conserved genes, which are specifically accessible and expressed during multicellular states. We validated that four of these genes, including the H3K27 deacetylase hdaD, are necessary and that an SMC-like gene, smcl1, is sufficient for multicellularity. These results highlight the importance of epigenetics in reorganizing chromatin architecture to facilitate the evolution of multicellularity.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterLindsay Pino
SpeciesList scientific name: Dictyostelium discoideum AX2; NCBI TaxID: 366501;
ModificationListMethyl; Propionyl; Delta:H(8)C(6)O(2); Acetyl
InstrumentQ Exactive HF; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-17 19:59:50ID requested
12021-02-19 06:23:11announced
22021-06-15 09:40:49announced: PubMed link added
Publication List
Wang SY, Pollina EA, Wang IH, Pino LK, Bushnell HL, Takashima K, Fritsche C, Sabin G, Garcia BA, Greer PL, Greer EL, Role of epigenetics in unicellular to multicellular transition in Dictyostelium. Genome Biol, 22(1):134(2021) [pubmed]
Keyword List
submitter keyword: epigenetics, histone, multicellularity
Contact List
Lindsay Pino
contact affiliationUniversity of Pennsylvania
contact emaillindsay.pino@pennmedicine.upenn.edu
lab head
Lindsay Pino
contact affiliationUniversity of Pennsylvania
contact emaillindsay.pino@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI