PXD024216

PXD024216 is an original dataset announced via ProteomeXchange.

Title	Shotgun-based Proteomics of Extracellular Vesicles in Alzheimer’s Disease Reveals Biomarkers Involved in Immunological and Coagulation Pathways
Description	Alzheimer’s dementia (AD) is the most common form of dementia and without readily available clinical biomarkers. Blood-derived proteins are routinely used for diagnostics, however, comprehensive plasma profiling is challenging due to the large dynamic range in protein concentrations. Extracellular vesicles (EVs) can cross the blood-brain barrier and may provide a source for AD related biomarkers. We investigated plasma-derived EV proteins for biomarkers towards AD diagnostics from 10 AD patients, 10 Mild Cognitive Impairment (MCI) patients, and 9 healthy controls (Con) using liquid chromatography - tandem mass spectrometry (LC-MS/MS). EV enrichment was performed using a double centrifugation of 100,000 × g, 1h, 4°C with a wash of the pellet. Some AD patients presented with highly elevated FXIIIA1 (log2 FC: 4.6, p-value: 0.005) and FXIIIB (log2 FC: 4.9, p-value: 0.018). A group of proteins was identified discriminating Con and AD (AUC: 0.91, CI: 0.67-1.00) with ORM2 (AUC: 1.00, CI: 1.00-1.00), RBP4 (AUC: 0.99, CI: 0.95-1.00), and HYDIN (AUC: 0.89, CI: 0.72-1.00) found especially relevant for AD. This indicates that EVs provide an easily accessible matrix for possible biomarkers for AD. Some of the MCI patients, with similar protein profiles as the AD group progressed to AD within a 2-year timespan.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_15:42:09.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Ellegaard Nielsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-17 06:43:21	ID requested
⏵ 1	2022-02-16 15:42:10	announced

Nielsen JE, Honor, é B, Vesterg, å, rd K, Maltesen RG, Christiansen G, B, ø, ge AU, Kristensen SR, Pedersen S, Shotgun-based proteomics of extracellular vesicles in Alzheimer's disease reveals biomarkers involved in immunological and coagulation pathways. Sci Rep, 11(1):18518(2021) [pubmed]

submitter keyword: Alzheimer, Mild Cognitive Impairment, Plasma, Extracellular Vesicles, Biomarkers, Blood, LC-MS/MS, Shotgun proteomics

Bent Honoré
contact affiliation	Department of Biomedicine, Aarhus University, Denmark
contact email	bh@biomed.au.dk
lab head
Jonas Ellegaard Nielsen
contact affiliation	Department of Clinical Medicine, Aalborg University, Aalborg, Denmark
contact email	jellegaard@dcm.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024216
     1. Label: PRIDE project
     2. Name: Shotgun-based Proteomics of Extracellular Vesicles in Alzheimer’s Disease Reveals Biomarkers Involved in Immunological and Coagulation Pathways