PXD024211

PXD024211 is an original dataset announced via ProteomeXchange.

Title	USP32 regulates non-proteolytic LAMTOR1 ubiquitination and Ragulator function
Description	Ubiquitination is among the most prevalent post-translational modifications regulating a great number of cellular functions, and defects in such processes contribute to many diseases. Deubiquitinating enzymes (DUBs) are essential to control the precise outcome of ubiquitin signals. The ubiquitin-specific protease 32 (USP32) differs from all other DUBs as it comprises in addition to its catalytic USP domain and the DUSP domain also multiple EF hands and a C-terminal prenylation site. This unique domain architecture offers various features for the regulation of specific signaling processes by USP32. To better understand the cellular function of USP32, we performed SILAC-based quantitative ubiquitinome analyses to determine potential USP32 substrates. We found proteins involved in endosomal trafficking as well as lysosomal proteins with regulated diGly sites in USP32 knockout (KO) cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:47.070.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Bonn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-16 23:04:55	ID requested
1	2022-12-16 12:58:39	announced
⏵ 2	2023-11-14 08:24:49	announced	2023-11-14: Updated project metadata.

Hertel A, Alves LM, Dutz H, Tascher G, Bonn F, Kaulich M, Dikic I, Eimer S, Steinberg F, Bremm A, USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction. Cell Rep, 41(10):111653(2022) [pubmed]

submitter keyword: Ubiquitin, diGly, Lysosome

Anja Bremm
contact affiliation	Institute of Biochemistry II, University Hospital, Goethe University, Frankfurt, Germany
contact email	bremm@em.uni-frankfurt.de
lab head
Florian Bonn
contact affiliation	University of Frankfurt
contact email	flobonn@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024211
     1. Label: PRIDE project
     2. Name: USP32 regulates non-proteolytic LAMTOR1 ubiquitination and Ragulator function