PXD024193

PXD024193 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of C-mannosyltransferase-deficient hiPSCs
Description	C-mannosylation is a modification of tryptophan residues with a single mannose effecting protein folding, secretion and/or function. To date, only few proteins have been proven to be C-mannosylated and studies aiming at global assessment of protein C-mannosylation from cells or tissues are scarce. In order to interrogate the C-mannosylome of human induced pluripotent stem cells (hiPSCs), we made use of the common finding that C-mannosylation is important for protein secretion. Thus we compared the secretomes of C-mannosyltransferase-deficient DPY19L1 and DPY19L3 mutants to their parental wild-type hiPSCs by mass-spectrometry-based quantitative proteomics. Secretion of numerous proteins was reduced in the mutants.
HostingRepository	PRIDE
AnnounceDate	2021-06-18
AnnouncementXML	Submission_2021-06-18_00:00:33.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karsten Cirksena
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-15 23:59:06	ID requested
⏵ 1	2021-06-18 00:00:34	announced

Cirksena K, H, ü, tte HJ, Shcherbakova A, Thumberger T, Sakson R, Weiss S, Jensen LR, Friedrich A, Todt D, Kuss AW, Ruppert T, Wittbrodt J, Bakker H, Buettner FFR, The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development. Mol Cell Proteomics, 20():100092(2021) [pubmed]

submitter keyword: hiPSCs, Secretomics, C-mannosylation, DPY19L1, DPY19L3, TSR

Falk Büttner
contact affiliation	Hannover Medical School Institute for Clinical Biochemistry (OE 4340) Carl-Neuberg-Strasse 1 Building J03, Level 04, Room 1291 (Office), Room 1250 (Lab) 30625 Hannover
contact email	buettner.falk@mh-hannover.de
lab head
Karsten Cirksena
contact affiliation	Hannover Medical School
contact email	cirksena.karsten@mh-hannover.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024193

PRIDE project URI

• PRIDE
  1. PXD024193
     1. Label: PRIDE project
     2. Name: Secretome analysis of C-mannosyltransferase-deficient hiPSCs