PXD024190

PXD024190 is an original dataset announced via ProteomeXchange.

Title	Proteomic landscape of multi-layered breast cancer internal tumor heterogeneity
Description	Breast cancer often presents with a high degree of intratumor heterogeneity, resulting in therapy resistance and tumor relapse. Here, we investigated spatial intratumor heterogeneity by integrating histopathological analyses and mass spectrometry-based proteomics. Using multilayered heterogeneity scoring, we identified the proteomic determinants of tumor heterogeneity and associated them with clinical and functional tumor characteristics. We found that molecular subtypes present lower heterogeneity with higher tumor grade, and demonstrate distinct subtype-specific profiles. However, even homogeneous tumors present high degrees of proteomic diversity, associated with proliferative and immune processes. Interestingly, tumor regions from molecularly-heterogeneous tumors, irrespective of their molecular subtype showed high proteomic similarity with enriched glutathione metabolism and proline biosynthesis pathways. Taken together the proteomic analyses revealed the association of internal tumor heterogeneity with cancer progression and immune selection, which can serve as a platform to decipher mechanisms underlying cancer evolution and therapy resistance.
HostingRepository	PRIDE
AnnounceDate	2025-08-11
AnnouncementXML	Submission_2025-08-10_17:15:21.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Geiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-15 23:46:29	ID requested
⏵ 1	2025-08-10 17:15:21	announced

Dataset with its publication pending

submitter keyword: breast cancer, proteomics,Intratumor heterogeneity, mass spectrometry

Tamar Geiger
contact affiliation	Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel-Aviv 6997801, Israel
contact email	geiger@tauex.tau.ac.il
lab head
Tamar Geiger
contact affiliation	Weizmann Institute of Science
contact email	geiger@tauex.tau.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD024190

PRIDE project URI

• PRIDE
  1. PXD024190
     1. Label: PRIDE project
     2. Name: Proteomic landscape of multi-layered breast cancer internal tumor heterogeneity