PXD024182

PXD024182 is an original dataset announced via ProteomeXchange.

Title	Modulation of rumen microbes through extracellular vesicle released by the rumen fluke Calicophoron daubneyi
Description	Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging parasite of livestock with many aspects of its underpinning biology yet to be resolved. This research is the first in-depth investigation of EVs released by adult C. daubneyi. EVs were successfully isolated using both differential centrifugation and size exclusion chromatography (SEC), and morphologically characterised though transmission electron microscopy (TEM). EV protein components were characterised using a GeLC approach allowing the elucidation of comprehensive proteomic profiles for both their soluble protein cargo and surface membrane bound proteins yielding a total of 378 soluble proteins identified
HostingRepository	PRIDE
AnnounceDate	2021-08-05
AnnouncementXML	Submission_2021-08-05_02:27:32.310.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathan Allen
SpeciesList	scientific name: Calicophoron daubneyi; NCBI TaxID: 300641;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-15 04:15:15	ID requested
⏵ 1	2021-08-05 02:27:33	announced

Allen NR, Taylor-Mew AR, Wilkinson TJ, Huws S, Phillips H, Morphew RM, Brophy PM, . Front Cell Infect Microbiol, 11():661830(2021) [pubmed]

submitter keyword: Extracellular vesicles, Calicophoron daubneyi, Gel-based proteomics, Proteome, Helminth

Russ M Morphew
contact affiliation	Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Edward Llwyd Z2.01.
contact email	rom@aber.ac.uk
lab head
Nathan Allen
contact affiliation	Aberystwyth University
contact email	nra3@aber.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024182

PRIDE project URI

• PRIDE
  1. PXD024182
     1. Label: PRIDE project
     2. Name: Modulation of rumen microbes through extracellular vesicle released by the rumen fluke Calicophoron daubneyi