PXD024169

PXD024169 is an original dataset announced via ProteomeXchange.

Title	Comparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics
Description	In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run identified about 9,200 proteins from 50 µg of Arabidopsis protein digest. The micro-flow LC-MS/MS system based ssDDA proteomics can be widely used for routine proteomics analysis of large cohort samples. We compared our micro data with the state of art nano LC data.
HostingRepository	PRIDE
AnnounceDate	2021-07-02
AnnouncementXML	Submission_2021-07-01_23:03:47.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yangyang Bian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-14 23:41:52	ID requested
1	2021-07-01 07:51:39	announced
⏵ 2	2021-07-01 23:03:48	announced	2021-07-02: Updated project metadata.

10.1021/ACS.ANALCHEM.1C00738;

Bian Y, The M, Giansanti P, Mergner J, Zheng R, Wilhelm M, Boychenko A, Kuster B, 000 Proteins from Cell Lines and Tissues by Single-Shot Microflow LC-MS/MS. Anal Chem, 93(25):8687-8692(2021) [pubmed]

Hebert AS, Prasad S, Belford MW, Bailey DJ, McAlister GC, Abbatiello SE, Huguet R, Wouters ER, Dunyach JJ, Brademan DR, Westphall MS, Coon JJ, Comprehensive Single-Shot Proteomics with FAIMS on a Hybrid Orbitrap Mass Spectrometer. Anal Chem, 90(15):9529-9537(2018) [pubmed]

submitter keyword: Single Shot Proteomics, Nano-flow LC,Micro-flow LC

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technische Universitaet Muenchen
contact email	kuster@tum.de
lab head
Yangyang Bian
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	yangyang.bian@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024169

PRIDE project URI

• PRIDE
  1. PXD024169
     1. Label: PRIDE project
     2. Name: Comparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics