PXD024164

PXD024164 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of breast EMT cell models reveal UGDH is upregulated during EMT and holds the potential as a clinical target
Description	Epithelial–mesenchymal transition (EMT) is known to be involved in cancer metastasis and pathogenesis. Metabolic re-wiring is now considered to be one of the hallmarks of cancer. In this study, we studied the metabolic changes during EMT in three breast EMT cell models with a proteomic approach. The study showed well-known changes during EMT including CDH1, CDH2, VIM, LGALS1, SERPINE1 and PKP3. It also suggested a list of dysregulated metabolic enzymes: SORD, TSTA3, FDFT1 and UGDH. UGDH had the biggest change and is associated with patient survival. We further studied the role of UGDH in EMT and found out knockdown of UGDH with siRNA can decrease the intracellular glycerophosphocholine level and it can also slow cell proliferation and invasion in mesenchymal cells. Besides, knockdown of UGDH downregulated the expression of a well-known EMT marker, SNAI1. PDGFRB is highly expressed in mesenchymal cells and PDGFD is highly secreted from mesenchymal cells. Knockdown of PDGFRB with siRNA downregulated UGDH potentially via NFkB.
HostingRepository	PRIDE
AnnounceDate	2022-01-04
AnnouncementXML	Submission_2022-01-04_00:11:29.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-12 06:48:01	ID requested
⏵ 1	2022-01-04 00:11:29	announced

Wang Q, Karvelsson ST, Johannsson F, Vilhjalmsson AI, Hagen L, de Miranda Fonseca D, Sharma A, Slupphaug G, Rolfsson O, UDP-glucose dehydrogenase expression is upregulated following EMT and differentially affects intracellular glycerophosphocholine and acetylaspartate levels in breast mesenchymal cell lines. Mol Oncol, 16(9):1816-1840(2022) [pubmed]

submitter keyword: breast, EMT, cell model, UGDH, upregulated, biomarker

Professor Óttar Rolfsson
contact affiliation	Department of Biochemistry and Molecular Biology & The Center for Systems Biology University of Iceland Sturlugata 8, 101 Reykjavik Iceland.
contact email	ottarr@hi.is
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD024164

PRIDE project URI

• PRIDE
  1. PXD024164
     1. Label: PRIDE project
     2. Name: Proteomic analysis of breast EMT cell models reveal UGDH is upregulated during EMT and holds the potential as a clinical target