PXD024149

PXD024149 is an original dataset announced via ProteomeXchange.

Title	The Anti-cancer Factor Quinolinone Inhibitors Disrupt Spindles and the Interactions between LSF and Microtubule-associated Proteins
Description	Identification of mitotic BioLSF-interacting proteins whose association is sensitive to Factor Quinolinone Inhibitor 1 (FQI1): The dataset is comprised of two independent experimental replicates, with each biological replicate consisting of runs of 4 samples. For each set, the four protein samples were generated from cells in mitosis, in which cells lacked, expressed basal levels, or expressed induced levels of a biotinylated, tagged LSF protein (BioLSF). Finally, cells with induced BioLSF levels were treated for one hour in mitosis with either FQI1 or vehicle (DMSO). Proteins purified on streptavidin beads were analyzed by mass spectrometry. More specifically, the four runs in each set derived from: 1) DLD-1 Flp-In™ T-REx™ TIR1 parental cells treated with vehicle (Parent_DMSO) as a negative control, 2) DLD-1 Flp-In™ T-REx™ TIR1 BioLSF cells with only leaky, basal BioLSF expression (BioLSF_DMSO), 3) DLD-1 Flp-In™ T-REx™ TIR1 BioLSF cells with induced BioLSF expression (BioLSF_DOX), and 4) DLD-1 Flp-In™ T-REx™ TIR1 BioLSF cells with induced BioLSF expression that were treated with FQI1 in mitosis (BioLSF_DOX_FQI1).
HostingRepository	PRIDE
AnnounceDate	2022-05-26
AnnouncementXML	Submission_2022-05-26_08:19:41.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulla Hansen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-11 22:56:24	ID requested
⏵ 1	2022-05-26 08:19:42	announced

Dataset with its publication pending

submitter keyword: Mitosis, LSF, TFCP2, FQI1, protein-protein interactions

Ulla Hansen
contact affiliation	Department of Biology Boston University Boston, MA, USA
contact email	uhansen@bu.edu
lab head
Ulla Hansen
contact affiliation	Boston University
contact email	uhansen@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD024149

PRIDE project URI

• PRIDE
  1. PXD024149
     1. Label: PRIDE project
     2. Name: The Anti-cancer Factor Quinolinone Inhibitors Disrupt Spindles and the Interactions between LSF and Microtubule-associated Proteins