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PXD024146

PXD024146 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReliable identification of protein-protein interactions by crosslinking mass spectrometry_AP-MS
DescriptionQuantitative proteomics dataset on soluble, affinity-enriched proteins from SPA-pulldowns in E. coli K12, on rpoB-SPA / nusG-SPA / yacL-SPA. This data was used to identify proteins for enrichment analysis and subsequent crosslinking mass spectrometry search (DB creation). Cleared E. coli lysate was added to anti-FLAG M2 beads to isolate binders to rpoB (RNA polymerase), nusG or yacL (RNA polymerase binders. Elution was accomplished via TEV protease cleavage. A mock K12 E.coli lysate ETV elution was used to assess protein enrichment in the obtained (specific) eluates. The proteins in each fraction were precipitated using acetone, derivatized and proteolysed in-solution. Each sample was acquired as injection triplicate via LC-MS on Q Exactive HF and the raw data searched with MaxQuant with LFQ and iBAQ quantitation enabled. Further processing was done using Perseus.
HostingRepositoryjPOST
AnnounceDate2021-04-14
AnnouncementXMLSubmission_2022-09-18_04:07:51.822.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLudwig Sinn
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListalpha-amino acetylated residue; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-11 10:09:31ID requested
12021-04-14 01:44:01announced
22022-09-18 04:07:52announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: AP-MS, crosslinking, mass spectrometry, FDR, protein-protein interaction, E. coli
Contact List
Prof. Dr. Juri Rappsilber
lab head
Ludwig Sinn
contact affiliationTechnische Universit├Ąt Berlin, Bioanalytics, Prof. Juri Rappsilber
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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