PXD024140

PXD024140 is an original dataset announced via ProteomeXchange.

Title	Multi-omic analyses 1 in Abyssinian cats with primary renal amyloid deposits
Description	The amyloidoses constitute a group of diseases occurring in humans and animals that are characterized by abnormal deposits of aggregated proteins in organs, affecting their structure and function. In the Abyssinian cat breed, a familial form of renal amyloidosis has been described. In this study, multi-omics analyses were applied and integrated to explore some aspects of the unknown pathogenetic processes in cats. Whole-genome sequences of two affected Abyssinians and 195 controls of other breeds (part of the 99 Lives initiative) were screened to prioritize potential disease-associated variants. Proteome and miRNAome from formalin-fixed paraffin-embedded kidney specimens of fully necropsied Abyssinian cats, three affected and three non-amyloidosis affected were characterized. While the trigger of the disorder remains unclear, overall, i) 35,960 genomic variants were detected; ii) 215 and 56 proteins were identified as exclusive or overexpressed in the affected and control kidneys, respectively; iii) 60 miRNAs were differentially expressed, 20 of which are newly described. With omics data integration, the general conclusions are:i) the familial amyloid renal form in Abyssinians is not a simple monogenic trait ii) amyloid deposition is not triggered by mutated amyloidogenic proteins but is a mix of proteins codified by wild-type genes iii) the form is biochemically classifiable as AA amyloidosis.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-10_00:24:26.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simona Nonnis
SpeciesList	scientific name: Felis catus; NCBI TaxID: 9685;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-11 07:59:23	ID requested
⏵ 1	2021-09-10 00:24:27	announced

Genova F, Nonnis S, Maffioli E, Tedeschi G, Strillacci MG, Carisetti M, Sironi G, Cupaioli FA, Di Nanni N, Mezzelani A, Mosca E, Helps CR, Leegwater PAJ, Dorso L, Longeri M, Buckley RM, Aberdein D, Alves PC, Andersson AO, Barsh GS, Bellone RR, Bergstr, ö, m TF, Boyko AR, Brockman JA, Casal ML, Castelhano MG, Distl O, Dodman NH, Ellinwood NM, Fogle JE, Forman OP, Garrick DJ, Ginns EI, Haase B, H, ä, ggstr, ö, m J, Harvey RJ, Hasegawa D, Hernandez I, Hyt, ö, nen MK, Kaukonen M, Kaelin CB, Kosho T, Leclerc E, Lear TL, Leeb T, Li RHL, Lohi H, Magnuson MA, Malik R, Mane SP, Munday JS, Murphy WJ, Pedersen NC, Peterson-Jones SM, Rothschild MF, Rusbridge C, Shapiro B, Stern JA, Swanson WF, Terio KA, Todhunter RJ, Warren WC, Wilcox EA, Wildschutte JH, Yu Y, Lyons LA, Multi-omic analyses in Abyssinian cats with primary renal amyloid deposits. Sci Rep, 11(1):8339(2021) [pubmed]

submitter keyword: Proteomics, LC-MS/MS

Simona Nonnis
contact affiliation	Department of Veterinary Medicine, University of Milan, Italy
contact email	simona.nonnis@unimi.it
lab head
Simona Nonnis
contact affiliation	University of Milan
contact email	simona.nonnis@unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024140
     1. Label: PRIDE project
     2. Name: Multi-omic analyses 1 in Abyssinian cats with primary renal amyloid deposits