PXD024139

PXD024139 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Intensity-based absolute quantification (iBAQ) of proteins in MDA-MB231
Description	To determine the absolute copy number of proteins in MDA-MB231 breast cancer cells, we employed IBAQ mediated absolute quantification of proteins based on (Schwanhäusser et al., Nature, 2011), with some modifications. Maqquant calculated iBAQ values were calibrated using spike-in standards, and used to calculate copy numbers for each identified protein within the dataset. Copy numbers for a total of 3,584 proteins were calculated in MDA-MB231 cells.
HostingRepository	PRIDE
AnnounceDate	2021-05-14
AnnouncementXML	Submission_2021-05-16_22:10:35.465.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Faraz Mardakheh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-11 07:59:23	ID requested
1	2021-05-14 06:32:39	announced
⏵ 2	2021-05-16 22:10:36	announced	2021-05-17: Updated publication reference for PubMed record(s): 33986153.

Publication List

Fish L, Khoroshkin M, Navickas A, Garcia K, Culbertson B, H, ä, nisch B, Zhang S, Nguyen HCB, Soto LM, Dermit M, Mardakheh FK, Molina H, Alarc, ó, n C, Najafabadi HS, Goodarzi H, A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements. Science, 372(6543):(2021) [pubmed]

Fish L, Khoroshkin M, Navickas A, Garcia K, Culbertson B, H, ä, nisch B, Zhang S, Nguyen HCB, Soto LM, Dermit M, Mardakheh FK, Molina H, Alarc, ó, n C, Najafabadi HS, Goodarzi H, A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements. Science, 372(6543):(2021) [pubmed]

Keyword List

submitter keyword: iBAQ
absolute protein quantification
breast cancer
MDA-MB231;

submitter keyword: iBAQ

absolute protein quantification

breast cancer

MDA-MB231;

Contact List

Faraz K. Mardakheh
contact affiliation	Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ UK.
contact email	f.mardakheh@qmul.ac.uk
lab head
Faraz Mardakheh
contact affiliation	Barts Cancer Institute
contact email	f.mardakheh@qmul.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024139
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024139

PRIDE project URI

Repository Record List

[ + ]