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PXD024117

PXD024117 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEndogenous THBS1 is C-mannosylated by human induced pluripotent stem cells.
DescriptionWe used a human thrombospondin-1 (THBS1) fragment comprising thrombospondin type 1 repeats (TSRs) 1 to 3, which was recombinantly expressed in HEK 293T cells and digested with AspN to determine chromatographic retention times and to optimise MS parameters. We detected two peptides from TSR2 and TSR3 containing the consensus site for C-mannosylation in their non-, mono- and di-C-mannosylated state. These peptides – irrespective of C-mannosylation - were detected at less than 3 % of the intensity of two other THBS-derived peptides which do not contain a WxxW motif (Figure 3A). With the knowledge of the specific chromatographic retention times and the intensity patterns of fragment ions for the two peptides containing the WxxW sites for C-mannosylation from the recombinant THBS1, we now analysed a native sample of hiPSC-derived proteins from cell culture supernatants by MRM. Indeed we detected the most intense fragment ions for the peptide DACPINGGWGPWSPW with one or two C-mannoses at the expected elution times and with the expected intensity profiles (Figure 3B).
HostingRepositoryPanoramaPublic
AnnounceDate2021-05-12
AnnouncementXMLSubmission_2021-05-12_11:02:38.614.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRoman Sakson
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListHex; Carbamidomethyl
InstrumentQTRAP 5500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-10 09:04:15ID requested
12021-05-12 11:02:39announced
Publication List
Cirksena K, Hütte HJ, Shcherbakova A, Thumberger T, Sakson R, Weiss S, Jensen LR, Friedrich A, Todt D, Kuss AW, Ruppert T, Wittbrodt J, Bakker H, Buettner FFR, The C-Mannosylome of Human Induced Pluripotent Stem Cells Implies a Role for ADAMTS16 C-Mannosylation in Eye Development. Mol Cell Proteomics, 20():100092(2021) [pubmed]
Keyword List
submitter keyword: hiPSC, TSR, thrombospondin, C-Mannosylation
Contact List
Falk F. R. Buettner
contact affiliationInstitute of Clinical Biochemistry, Hannover Medical School, 30625 Hannover, Germany
contact emailBuettner.Falk@mh-hannover.de
lab head
Roman Sakson
contact affiliationZentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany
contact emailroman.sakson@gmx.net
dataset submitter
Full Dataset Link List
Panorama Public dataset URI