PXD024115

PXD024115 is an original dataset announced via ProteomeXchange.

Title	Comparison of preservation and storage methods for metaproteomics of intestinal microbiome samples
Description	The aim of this study was to develop a suitable method to preserve fecal samples for metaproteomics analyses when flash-freezing is not an option. Fecal samples were collected from conventional adult C57BL/6 mice and combined into a fecal master mix. The fecal master mix was then split into 48 subsamples that were subjected to different preservation treatments. The following six preservation methods were tested: flash-freezing in liquid nitrogen followed by storage at -80°C, immersion in RNAlater® and storage at room temperature, immersion in RNAlater® and immediate storage at -80°C, immersion in 95% ethanol and storage at room temperature, immersion in a RNAlater-like buffer “NAP buffer” and storage at room temperature, and immersion in an autoclaved RNAlater-like buffer “Autoclaved NAP buffer” and storage at room temperature. Proteins were extracted from the samples after being stored for 1 and 4 weeks. There were 4 replicates per treatment and time-point. Samples were analyzed by LC-MS/MS and the data were analyzed with Proteome Discoverer against a large database of mouse microbiota protein sequences.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2022-01-10_11:18:13.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angie Mordant
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: mouse gut metagenome; NCBI TaxID: 410661;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-10 08:04:23	ID requested
1	2021-11-25 07:57:06	announced
⏵ 2	2022-01-10 11:18:14	announced	2022-01-10: Updated publication reference for PubMed record(s): 34908431.

Mordant A, Kleiner M, Evaluation of Sample Preservation and Storage Methods for Metaproteomics Analysis of Intestinal Microbiomes. Microbiol Spectr, 9(3):e0187721(2021) [pubmed]

submitter keyword: 1D-LC

mass spectrometry

MS/MS

metaproteomics

metaproteome

microbiota

microbiome

gut

intestinal

fecal

mouse

storage

preservation.

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Angie Mordant
contact affiliation	North Carolina State University
contact email	almordan@ncsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024115

PRIDE project URI

• PRIDE
  1. PXD024115
     1. Label: PRIDE project
     2. Name: Comparison of preservation and storage methods for metaproteomics of intestinal microbiome samples