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PXD024106

PXD024106 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAcid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesiclesAcid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesicles
DescriptionAcid sphingomyelinase (ASM) inhibitors, which are clinically used as anti-depressants for ~60 years, have recently been shown to enhance stroke recovery in rodents. Using mice and cerebral microvascular endothelial cells exposed to ischemia/reperfusion (I/R) we show that the antidepressants amitriptyline, fluoxetine and desipramine induce angiogenesis in an ASM-dependent way by releasing small extracellular vesicles (sEVs) from endothelial cells, which have bona fide characteristics of exosomes and which, similar to sEVs released during I/R, promote angiogenesis. Post-I/R, ASM inhibition elicits a profound brain remodeling response with increased blood-brain barrier integrity, reduced brain leukocyte infiltrates and increased neuronal survival. The ASM inhibitor-mediated release of sEVs has disclosed an elegant target, via which stroke recovery can be amplified. Key words: Antidepressant, ceramide, exosome, focal cerebral ischemia, middle cerebral artery occlusion, sphingomyelin, stroke recovery
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:54:32.087.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFiorella Andrea Solari
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-10 03:10:12ID requested
12022-10-13 19:23:24announced
22023-11-14 07:54:33announced2023-11-14: Updated project metadata.
Publication List
Mohamud Yusuf A, Hagemann N, Zhang X, Zafar M, Hussner T, Bromkamp C, Martiny C, Tertel T, B, ö, rger V, Schumacher F, Solari FA, Hasenberg M, Kleinschnitz C, Doeppner TR, Kleuser B, Sickmann A, Gunzer M, Giebel B, Kolesnick R, Gulbins E, Hermann DM, Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles. Basic Res Cardiol, 117(1):43(2022) [pubmed]
Keyword List
submitter keyword: Antidepressants,exosomes, label free quantification, LC-MS/MS
Contact List
Prof. Dr Albert Sickmann sickmann@isas.de>
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact emailsickmann@isas.de
lab head
Fiorella Andrea Solari
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact emailfiorella.solari@isas.de
dataset submitter
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Dataset FTP location
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