PXD024106 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Acid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesiclesAcid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesicles |
Description | Acid sphingomyelinase (ASM) inhibitors, which are clinically used as anti-depressants for ~60 years, have recently been shown to enhance stroke recovery in rodents. Using mice and cerebral microvascular endothelial cells exposed to ischemia/reperfusion (I/R) we show that the antidepressants amitriptyline, fluoxetine and desipramine induce angiogenesis in an ASM-dependent way by releasing small extracellular vesicles (sEVs) from endothelial cells, which have bona fide characteristics of exosomes and which, similar to sEVs released during I/R, promote angiogenesis. Post-I/R, ASM inhibition elicits a profound brain remodeling response with increased blood-brain barrier integrity, reduced brain leukocyte infiltrates and increased neuronal survival. The ASM inhibitor-mediated release of sEVs has disclosed an elegant target, via which stroke recovery can be amplified. Key words: Antidepressant, ceramide, exosome, focal cerebral ischemia, middle cerebral artery occlusion, sphingomyelin, stroke recovery |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:54:32.087.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fiorella Andrea Solari |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-10 03:10:12 | ID requested | |
1 | 2022-10-13 19:23:24 | announced | |
⏵ 2 | 2023-11-14 07:54:33 | announced | 2023-11-14: Updated project metadata. |
Publication List
Mohamud Yusuf A, Hagemann N, Zhang X, Zafar M, Hussner T, Bromkamp C, Martiny C, Tertel T, B, ö, rger V, Schumacher F, Solari FA, Hasenberg M, Kleinschnitz C, Doeppner TR, Kleuser B, Sickmann A, Gunzer M, Giebel B, Kolesnick R, Gulbins E, Hermann DM, Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles. Basic Res Cardiol, 117(1):43(2022) [pubmed] |
Keyword List
submitter keyword: Antidepressants,exosomes, label free quantification, LC-MS/MS |
Contact List
Prof. Dr Albert Sickmann sickmann@isas.de> |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. |
contact email | sickmann@isas.de |
lab head | |
Fiorella Andrea Solari |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. |
contact email | fiorella.solari@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024106
- Label: PRIDE project
- Name: Acid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesiclesAcid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesicles