PXD024091

PXD024091 is an original dataset announced via ProteomeXchange.

Title	N-terminomics upon Arginyl-tRNA synthetase knock down
Description	Arginine is associated with inflammation, cancer, and amino acid signaling, in part through the mTORC1 pathway. In cell-based assays and in the mouse, we found that arginine regulates nuclear levels of arginyl-tRNA synthetase (ArgRS), and that arginine depletion and inflammation reduced nuclear ArgRS in vivo. In the nucleus, ArgRS interacted and co-localized with the spliceosomal Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) that is crucial for the formation of nuclear speckle condensates. ArgRS mRNA silencing changed specific splice junction usages, and these inversely correlated with SRRM2-induced usage changes of the same junctions. The prominently affected genes encompassed components of the mTORC1 pathway and showed ArgRS, arginine, and SRRM2 are tied together as upstream regulators of nuclear condensate trafficking related to the physiological response to inflammatory injury. This study is the first to demonstrate a regulatory role for an aaRS in shaping nuclear condensate dynamics and RNA splicing.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:57.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haissi Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dimethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-09 10:30:14	ID requested
1	2023-04-14 13:38:15	announced
⏵ 2	2023-11-14 08:51:57	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: tRNA, aminoacyl-tRNA synthetase, splicing, N-terminomics

Paul Schimmel
contact affiliation	Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
contact email	schimmel@scripps.edu
lab head
Haissi Cui
contact affiliation	Scripps Research
contact email	hacui@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD024091

PRIDE project URI

• PRIDE
  1. PXD024091
     1. Label: PRIDE project
     2. Name: N-terminomics upon Arginyl-tRNA synthetase knock down