PXD024081

PXD024081 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome-wide analysis of elongating nascent polypeptide chains – Nt-acetylation analysis
Description	Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse labeling with puromycin, and stable isotope-labeled amino acids to analyze thousands of NPCs. Our approach enables global analyses of translational responses and co-translational modifications, providing a framework for dissecting co-translational regulations proteome-wide. HeLa cells were treated with either CHX or DMSO for 2 hr in the presence of puromycin and corresponding SILAC amino acid pairs. By combining pSNAP with low pH strong cation exchange (SCX) chromatography, we enriched Nt-acetylated peptides that were eluted earlier in SCX fractionation due to the loss of positive charge at their N-terminal ends. Immunoprecipitation of puromycylated proteins was performed in triplicates.
HostingRepository	jPOST
AnnounceDate	2022-02-09
AnnouncementXML	Submission_2024-03-12_19:37:17.384.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-09 05:46:16	ID requested
1	2022-02-08 07:00:16	announced
2	2022-09-18 03:18:18	announced	2022-09-18: Updated FTP location.
⏵ 3	2024-03-12 19:37:18	announced	2024-03-13: Updated PubMed.

Publication List

Uchiyama J, Roy R, Wang DO, Morikawa K, Kawahara Y, Iwasaki M, Yoshino C, Mishima Y, Ishihama Y, Imami K, pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains. iScience, 25(7):104516(2022) [pubmed]

Uchiyama J, Roy R, Wang DO, Morikawa K, Kawahara Y, Iwasaki M, Yoshino C, Mishima Y, Ishihama Y, Imami K, pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains. iScience, 25(7):104516(2022) [pubmed]

Keyword List

submitter keyword: nascent polypeptide, puromycin, ribosome, translation

submitter keyword: nascent polypeptide, puromycin, ribosome, translation

Contact List

Yasushi Ishihama
lab head
Koshi Imami
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001087/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001087/