PXD024071

PXD024071 is an original dataset announced via ProteomeXchange.

Title	An efficient in-gel digestion method on a small amounts of protein sample
Description	We have verified each step of in-gel digestion. We reduced the sample processing time and labor by optimizing the following four points. 1. Using intact gel pieces. 2. Use 96-well filter and positive pressure manifold before digestion. 3. Extract digested peptide without acetonitrile 4. In order to minimize non-specific absorption, digest in a low-peptides-binding microtube not in the 96-well filter or low-peptides-binding 96-well multiplate.
HostingRepository	jPOST
AnnounceDate	2023-02-09
AnnouncementXML	Submission_2023-02-08_07:00:04.875.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keiko Kano
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 22:27:59	ID requested
⏵ 1	2023-02-08 07:00:05	announced

Dataset with its publication pending

submitter keyword: In-gel digestion, a small amount of protein sample

Keiko Kuwata
lab head
Keiko Kano
contact affiliation	Nagoya university
dataset submitter

jPOST dataset URI

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