PXD024066 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human mitochondrial protein import proteomics - Proteinase K control |
Description | Most mitochondrial proteins are encoded in the nucleus and require mitochondrial import after translation in the cytosol. A number of mutations in the mitochondrial protein import machinery have been shown to lead to human pathologies. However, a lack suitable tools to measure mitochondrial protein uptake has prevented determination of import rates across the mitochondrial proteome and identification of specific proteins affected by perturbation. Here, we introduce a pulsed-SILAC based proteomics approach that includes a booster signal to selectively increase the sensitivity for mitochondrial proteins, enabling dynamic analysis of mitochondrial protein uptake at the global scale. We applied this method to determine protein uptake kinetics and examined how inhibitors of different mitochondrial import machineries affect protein uptake in sub-mitochondrial compartments. Monitoring changes in translation and protein uptake upon mitochondrial membrane depolarization revealed that protein uptake was extensively modulated the import- and translation machineries, via activation of the integrated stress response. Strikingly, uptake changes were not uniform with three groups of protein identified that showed no changes in uptake, or changes driven by reduced translation or import capacity. This study provides with a quantitative proteomics method to monitor mitochondrial protein uptake at a global scale to provide with insight into uptake rearrangements upon perturbation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-20 |
AnnouncementXML | Submission_2021-12-20_03:54:51.728.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonas Michaelis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-08 08:48:29 | ID requested | |
⏵ 1 | 2021-12-20 03:54:52 | announced | |
Publication List
Sch, ä, fer JA, Bozkurt S, Michaelis JB, Klann K, M, ü, nch C, Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery. Mol Cell, 82(2):435-446.e7(2022) [pubmed] |
Keyword List
submitter keyword: human, mePROD, DDA, TMD, TMT, mitochondrial protein import |
Contact List
Christian Münch |
contact affiliation | Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany |
contact email | ch.muench@em.uni-frankfurt.de |
lab head | |
Jonas Michaelis |
contact affiliation | Goethe Universität Frankfurt am Main, Faculty of medicine, Institute of Biochemistry II, Münch Group |
contact email | michaelis@med.uni-frankfurt.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024066
- Label: PRIDE project
- Name: Human mitochondrial protein import proteomics - Proteinase K control