PXD024065

PXD024065 is an original dataset announced via ProteomeXchange.

Title	Interaction of Hsp70 Ssb1 and atypical Hsp70 Ssz1
Description	Our goal was to better understand the interaction between the Hsp70 Ssb1 and the atypical Hsp70 Ssz1, which we had uncovered by in vivo site-specific Bpa crosslinking. Ssz1 having Bpa incorporated was purified and incubated with purified Ssb1 and the J-domain protein Zuo1 during activation of photo cross-linking. After protein gel electrophoresis and protease digestion of excised bands, the resulting cross-linked peptides were analyzed by mass spectrometry and identified by StavroX.
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_07:33:12.309.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024065
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Greg Sabat
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 08:41:07	ID requested
⏵ 1	2021-08-26 07:33:12	announced

Dataset with its publication pending

submitter keyword: Yeast, photo crosslinking, Ssb1, Ssz1, mass spectrometry

Dr. Elizabeth A, Craig
contact affiliation	Department of Biochemistry, University of Wisconsin - Madison
contact email	ecraig@wisc.edu
lab head
Greg Sabat
contact affiliation	University of Wisconsin
contact email	gsabat@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024065

PRIDE project URI

• PRIDE
  1. PXD024065
     1. Label: PRIDE project
     2. Name: Interaction of Hsp70 Ssb1 and atypical Hsp70 Ssz1