PXD024061

PXD024061 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomic analysis of nitrogen starvation in Arabidopsis root reveals new aspects of H2S signaling by protein persulfidation
Description	Hydrogen sulfide (H2S)-mediated signaling pathways regulate many physiological and pathophysiological processes in mammalian and plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, and it is named protein Persulfidation. We have developed a comparative and label-free quantitative proteomic analysis approach for the detection of endogenous persulfidated proteins in Arabidopsis thaliana nitrogen-starved roots and carbon-starved leaves by using the tag-switch method. In this work, we have identified 5214 and 4691 unique proteins from root and leaf tissue, respectively, that represent almost a 13% of the entire annotate proteome in Arabidopsis. Bioinformatic analysis revealed that persulfidated cysteines are part of a wide range of biological functions, regulating important processes such as carbon metabolism, plant responses to abiotic and biotic stresses, plant growth and development, RNA translation and protein degradation. Quantitative analysis in both tissues showed important H2S-regulated metabolic processes through changes in the persulfidation level of key protein targets involved in ubiquitin-dependent protein degradation and autophagy, among others.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:57.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Zenezini Chiozzi
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue; L-cysteine persulfide; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 08:18:55	ID requested
1	2021-09-09 04:56:17	announced
2	2021-09-21 23:24:50	announced	2021-09-22: Updated publication reference for PubMed record(s): 33805243. 2021-09-22: Updated publication reference for DOI(s): 10.3390/ANTIOX10040508.
⏵ 3	2023-11-14 08:51:58	announced	2023-11-14: Updated project metadata.

Jurado-Flores A, Romero LC, Gotor C, S Signaling by Protein Persulfidation. Antioxidants (Basel), 10(4):(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: Hydrogen sulfide mediated signaling pathways

proteomics

arabidopsis thaliana

Luis C. Romero
contact affiliation	Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-US Avda Americo Vespucio 49, 41092-Sevilla, Spain
contact email	lromero@ibvf.csic.es
lab head
Riccardo Zenezini Chiozzi
contact affiliation	UCL Mass Spectrometry Science Technology Platform, Division of Biosciences, University College London, London
contact email	r.zenezinichiozzi@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024061

PRIDE project URI

• PRIDE
  1. PXD024061
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomic analysis of nitrogen starvation in Arabidopsis root reveals new aspects of H2S signaling by protein persulfidation