PXD024058

PXD024058 is an original dataset announced via ProteomeXchange.

Title	Tianlongkechuanling Inhibits Pulmonary Fibrosis Through Down-Regulation Of Arginase-Ornithine Pathway
Description	Background: Pulmonary Fibrosis (PF) is an interstitial lung disease of unknown etiology, characterized by excessive accumulation of extracellular matrix in the lungs, which disrupts the structure and gas exchange of the alveoli. There are only two approved therapies for PF, nintedanib (Nib) and pirfenidone. Therefore, the use of Chinese medicine for PF is attracting attention. Tianlongkechuanling (TL) is an effective Chinese formula that has been applied clinically to alleviate PF, which can enhance lung function and quality of life. Purpose: The potential effects and specific mechanisms of TL have not been fully explored, yet. In the present study, proteomics was performed to explore the therapeutic protein targets of TL on Bleomycin (BLM)-induced Pulmonary Fibrosis. Method: BLM-induced PF mice models were established. Hematoxylineosin staining and Masson staining were used to analyze histopathological changes and collagen deposition. To screen the differential proteins expression between the Control, BLM, BLM+TL and BLM+ Nib (BLM+ Nintedanib) groups, quantitative proteomics was performed using tandem mass tag (TMT) labeling with nanoLC-MS/MS [nano liquid chromatographymass spectrometry]). Changes in the profiles of the expressed proteins were analyzed using the bioinformatics tools Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The protein–protein interactions (PPI) were established by STRING. Expressions of α-smooth muscle actin (α-SMA), Collagen I (Col1a1), Fibronectin (Fn1) and enzymes in arginase-ornithine pathway were detected by Western blot or RT-PCR.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_14:25:22.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lili Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 07:16:31	ID requested
⏵ 1	2022-02-16 14:25:23	announced

Zhang L, Qu S, Wang L, Wang C, Yu Q, Zhang Z, Diao Y, Zhang B, Li Y, Shi Y, Wang P, Tianlongkechuanling Inhibits Pulmonary Fibrosis Through Down-Regulation of Arginase-Ornithine Pathway. Front Pharmacol, 12():661129(2021) [pubmed]

submitter keyword: proteomics, Tianlongkechuanling, pulmonary fibrosis, arginase, arginase-ornithine pathway

Yuanyuan Shi
contact affiliation	1 School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.2 Shenzhen Research Institute, Beijing University of Chinese Medicine, Shenzhen, China.
contact email	yshi@bucm.edu.cn
lab head
Lili Zhang
contact affiliation	School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China
contact email	811216118@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024058
     1. Label: PRIDE project
     2. Name: Tianlongkechuanling Inhibits Pulmonary Fibrosis Through Down-Regulation Of Arginase-Ornithine Pathway