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PXD024051 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation
DescriptionCells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII ubiquitylation at a single lysine (K1268) by the CRL4CSA ubiquitin ligase. How CRL4CSA is specifically directed toward the K1268 site is unknown. Here, we identify ELOF1 as the missing link that facilitates RNAPII ubiquitylation, a key signal for the assembly of downstream repair factors. This function requires its constitutive interaction with RNAPII close to the K1268 site, revealing ELOF1 as a specificity factor that interacts with and positions CRL4CSA for optimal RNAPII ubiquitylation. Drug-genetic interaction screening also reveals a CSB-independent compensatory pathway in which ELOF1 protects cells against DNA replication stress by preventing DNA damage-induced R-loops. Our study offers key insights into the molecular mechanisms of TCR and provides a genetic framework of the interplay between the transcriptional stress response and DNA replication.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomán González-Prieto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-08 07:11:18ID requested
12021-04-20 22:38:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TC-NER, RNA Polymerase II, transcription, DNA repair.
Contact List
Román González-Prieto
contact affiliationCell and Chemical Biology Leiden University Medical Center The Netherlands
contact emailR.Gonzalez_Prieto@lumc.nl
lab head
Román González-Prieto
contact affiliationLeiden University Medical Center (LUMC)
contact emailR.Gonzalez_Prieto@lumc.nl
dataset submitter
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Dataset FTP location
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