PXD024050

PXD024050 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Peptidomics of enteroendocrine cells and characterisation of potential effects of a novel preprogastrin derived-peptide on glucose tolerance in lean mice
Description	To analyse the peptidomics of mouse enteroendocrine cells (EECs) and human gastrointestinal (GI) tissue and identify novel gut derived peptides. High resolution nano-flow liquid chromatography mass spectrometry (LCMS) was performed on (i) flow-cytometry purified NeuroD1 positive cells from mouse and homogenised human intestinal biopsies, (ii) supernatants from primary murine intestinal cultures, (iii) intestinal homogenates from mice fed high fat diet. Candidate bioactive peptides were selected on the basis of species conservation, high expression/biosynthesis in EECs and evidence of regulated secretion in vitro. Candidate novel gut-derived peptides were chronically administered to mice to assess effects on food intake and glucose tolerance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:34.905.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024050
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Richard Kay
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 06:42:13	ID requested
1	2021-03-29 12:01:16	announced
2	2023-11-14 08:51:19	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:20:35	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD024050;
10.1016/J.PEPTIDES.2021.170532;

10.6019/PXD024050;

10.1016/J.PEPTIDES.2021.170532;

Keyword List

submitter keyword: Enteroendocrine cells
peptidomics
mass spectrometry
prohormones
granins
progastrin

submitter keyword: Enteroendocrine cells

peptidomics

mass spectrometry

prohormones

granins

progastrin

Contact List

Professor Fiona Gribble
contact affiliation	Wellcome Trust, MRC Institute of Metabolic Science, University of Cambridge.
contact email	fmg23@cam.ac.uk
lab head
Richard Kay
contact affiliation	University of Cambridge
contact email	rgk27@medschl.cam.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024050
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024050

PRIDE project URI

Repository Record List

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