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PXD024050

PXD024050 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeptidomics of enteroendocrine cells and characterisation of potential effects of a novel preprogastrin derived-peptide on glucose tolerance in lean mice
DescriptionTo analyse the peptidomics of mouse enteroendocrine cells (EECs) and human gastrointestinal (GI) tissue and identify novel gut derived peptides. High resolution nano-flow liquid chromatography mass spectrometry (LCMS) was performed on (i) flow-cytometry purified NeuroD1 positive cells from mouse and homogenised human intestinal biopsies, (ii) supernatants from primary murine intestinal cultures, (iii) intestinal homogenates from mice fed high fat diet. Candidate bioactive peptides were selected on the basis of species conservation, high expression/biosynthesis in EECs and evidence of regulated secretion in vitro. Candidate novel gut-derived peptides were chronically administered to mice to assess effects on food intake and glucose tolerance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:20:34.905.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD024050
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRichard Kay
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListamidated residue; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-08 06:42:13ID requested
12021-03-29 12:01:16announced
22023-11-14 08:51:19announced2023-11-14: Updated project metadata.
32024-10-22 05:20:35announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD024050;
10.1016/J.PEPTIDES.2021.170532;
Keyword List
submitter keyword: Enteroendocrine cells
peptidomics
mass spectrometry
prohormones
granins
progastrin
Contact List
Professor Fiona Gribble
contact affiliationWellcome Trust, MRC Institute of Metabolic Science, University of Cambridge.
contact emailfmg23@cam.ac.uk
lab head
Richard Kay
contact affiliationUniversity of Cambridge
contact emailrgk27@medschl.cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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