PXD024050 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptidomics of enteroendocrine cells and characterisation of potential effects of a novel preprogastrin derived-peptide on glucose tolerance in lean mice |
Description | To analyse the peptidomics of mouse enteroendocrine cells (EECs) and human gastrointestinal (GI) tissue and identify novel gut derived peptides. High resolution nano-flow liquid chromatography mass spectrometry (LCMS) was performed on (i) flow-cytometry purified NeuroD1 positive cells from mouse and homogenised human intestinal biopsies, (ii) supernatants from primary murine intestinal cultures, (iii) intestinal homogenates from mice fed high fat diet. Candidate bioactive peptides were selected on the basis of species conservation, high expression/biosynthesis in EECs and evidence of regulated secretion in vitro. Candidate novel gut-derived peptides were chronically administered to mice to assess effects on food intake and glucose tolerance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:20:34.905.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD024050 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Richard Kay |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | amidated residue; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-08 06:42:13 | ID requested | |
1 | 2021-03-29 12:01:16 | announced | |
2 | 2023-11-14 08:51:19 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:20:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Enteroendocrine cells |
peptidomics |
mass spectrometry |
prohormones |
granins |
progastrin |
Contact List
Professor Fiona Gribble |
contact affiliation | Wellcome Trust, MRC Institute of Metabolic Science, University of Cambridge. |
contact email | fmg23@cam.ac.uk |
lab head | |
Richard Kay |
contact affiliation | University of Cambridge |
contact email | rgk27@medschl.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024050
- Label: PRIDE project
- Name: Peptidomics of enteroendocrine cells and characterisation of potential effects of a novel preprogastrin derived-peptide on glucose tolerance in lean mice