PXD024031

PXD024031 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of small extracellular vesicles released from polarized MDCK cells
Description	Exosomes, important players in cell-cell communication, are small extracellular vesicles of endocytic origin. Although single cells are known to release various kinds of exosomes (referred to as exosomal heterogeneity), very little is known about the mechanisms by which they are produced and released. Here, we established methods of studying exosomal heterogeneity by using polarized epithelial cells and showed that distinct types of small extracellular vesicles (more specifically CD9- and CD63-positive, Annexin I-negative small extracellular vesicles, which we refer to as exosomes herein) are differentially secreted from the apical and basolateral sides of polarized epithelial cells. We also identify GPRC5C (G protein-coupled receptor class C group 5 member C) as an apical-exosome-specific protein. We further demonstrate that basolateral exosome release depends on ceramide, whereas ALIX, an ESCRT (endosomal sorting complexes required for transport)-related protein, not the ESCRT machinery itself, is required for apical exosome release. Thus, two independent machineries, the ALIX–Syntenin1–Syndecan1 machinery (apical side) and the sphingomyelinase-dependent ceramide production machinery (basolateral side), are likely to be responsible for the polarized exosome release from epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2021-03-03
AnnouncementXML	Submission_2021-03-03_00:40:55.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mitsunori Fukuda
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-07 23:34:07	ID requested
⏵ 1	2021-03-03 00:40:55	announced

Dataset with its publication pending

submitter keyword: Canine, MDCK, extracellular vesicles

Mitsunori Fukuda
contact affiliation	Laboratory of Membrane Trafficking Mechanisms, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
contact email	nori@tohoku.ac.jp
lab head
Mitsunori Fukuda
contact affiliation	Tohoku University, Graduate School of Life Sciences
contact email	nori@tohoku.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024031

PRIDE project URI

• PRIDE
  1. PXD024031
     1. Label: PRIDE project
     2. Name: Proteomic analysis of small extracellular vesicles released from polarized MDCK cells