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PXD024029

PXD024029 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleShadow ORFs illuminated: long overlapping genes in Pseudomonas aeruginosa are translated and under purifying selection
DescriptionThe existence of overlapping genes (OLGs) with significant coding overlaps revolutionises our understanding of genomic complexity. We report two exceptionally long (957 nt and 1536 nt), evolutionarily novel, translated antisense open reading frames (ORFs) embedded within annotated genes in Pseudomonas aeruginosa. Both OLG pairs show features consistent with being genes and transcriptional signals as indicated by transcriptome sequencing. Translation of both OLGs was confirmed by ribosome profiling as well as mass spectrometry. Quantitative proteomics of samples taken during different phases of growth revealed regulation of protein abundances, implying biological functionality. Both OLGs are taxonomically highly restricted and likely arose by overprinting within the genus. Evidence for purifying selection further supports functionality. The here established OLGs are the longest yet proposed in prokaryotes and are the best attested in terms of translation and evolutionary constraint.
HostingRepositoryPanoramaPublic
AnnounceDate2021-02-08
AnnouncementXMLSubmission_2021-02-08_06:08:51.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristina Ludwig
SpeciesList scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-07 20:43:31ID requested
12021-02-08 06:08:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Overlapping genes, mass spectrometry, ribosome profiling, orphan genes, overprinting, de novo gene origin
Contact List
Christina Ludwig
contact affiliationBavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University Munich (TUM), Germany
contact emailtina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliationBavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University Munich (TUM), Germany
contact emailtina.ludwig@tum.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI