PXD024020

PXD024020 is an original dataset announced via ProteomeXchange.

Title	Intramolecular synergism and proteolytic control of bacteriophage endolysin PlySK1249
Description	Endolysins are peptidoglycan hydrolases produced at the end of the bacteriophage (phage) replication cycle to lyse the host cell. Gram-positive phages endolysins come in a variety of multi-modular forms that combine different catalytic domains and may have evolved to adapt to their bacterial hosts. However, the reason why phage can adopt endolysin with such complex multidomain architecture is for the moment not well understood. We used the Streptococcus dysgalactiae phage endolysin PlySK1249 as a model to study the implication of multi-domain architecture in phage-induced bacterial lysis and lysis regulation. The activity of the enzyme relied on a bacteriolytic amidase (Ami), a non-bacteriolytic L-Ala-D-Ala endopeptidase (CHAP) acting as a de-chaining enzyme and central LysM cell wall binding domain (CBD). Ami and CHAP synergized for peptidoglycan digestion and bacteriolysis in the native enzyme or when expressed individually and reunified in vitro. This cooperation could be modulated by bacterial cell wall-associated proteases, which specifically cleaved the two linkers connecting the different domains. While both catalytic domains were observed to act coordinately to optimize bacterial lysis, the CBD is expected to delay diffusion of the enzyme until proteolytic inactivation is achieved. As for certain autolysins, PlySK1249 cleavage by bacterial cell wall associated proteases might be an example of dual phage-bacterial regulation and mutual coevolution.
HostingRepository	PRIDE
AnnounceDate	2021-04-16
AnnouncementXML	Submission_2021-04-15_21:41:42.892.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024020
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Streptococcus dysgalactiae subsp. equisimilis SK1249; NCBI TaxID: 1000589; scientific name: Streptococcus agalactiae FSL S3-026; NCBI TaxID: 876138;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-05 03:42:21	ID requested
1	2021-04-15 00:39:08	announced
⏵ 2	2021-04-15 21:41:43	announced	2021-04-16: Updated project metadata.

10.1016/J.JBC.2021.100639;

Oechslin F, Menzi C, Moreillon P, Resch G, The multidomain architecture of a bacteriophage endolysin enables intramolecular synergism and regulation of bacterial lysis. J Biol Chem, 296():100639(2021) [pubmed]

submitter keyword: endolysin, proteolysis,Bacteriophage, LC-MS/MS

Frank Oechslin
contact affiliation	Department of Fundamental Microbiology, University of Lausanne, 1015 Lausanne, Switzerland.
contact email	Frank.Oechslin@gmail.com
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024020
     1. Label: PRIDE project
     2. Name: Intramolecular synergism and proteolytic control of bacteriophage endolysin PlySK1249