PXD024018

PXD024018 is an original dataset announced via ProteomeXchange.

Title	Kininogen–Nitric Oxide Signaling at Nearby Non-excited Acupoints After Long-term Stimulation
Description	Acupuncture treatment is based on acupoint stimulation; however, the biological basis is not understood. We stimulated one acupoint with catgut embedding (CEP) for 8 weeks and then used isobaric tags for relative and absolute quantitation to screen proteins with altered expression in adjacent acupoints of Sprague Dawley rats. The expression levels of 10 proteins, including kininogen (KNG), consistently changed >1.5-fold in all three acupoints in CEP versus sham CEP-treated rats. Eight-week, but not 3-day, CEP treatment enhanced KNG expression. The enhanced KNG expression among small vessels in the subcutaneous layer was revealed via immunofluorescence. Nitric oxide synthesis was enhanced similar to KNG. These findings uncover biological changes at acupoints and suggest the critical role of the KNG–nitric oxide signaling pathway in acupoint activation.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_14:18:44.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liyue Qin
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-05 03:08:28	ID requested
⏵ 1	2022-02-16 14:18:45	announced

Wang T, Zhu G, Qin L, Wang Q, She C, Xu D, Hu W, Luo K, Lei Y, Gong Y, Ghosh A, Ma D, Ding CL, Wang BY, Guo Y, Ma SS, Hattori M, Takagi Y, Ara K, Higuchi K, Li X, He L, Bai W, Ishida K, Li ST, Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innov, 1(3):100038(2021) [pubmed]

submitter keyword: KNG–nitric oxide signaling pathway，Acupoints， Long-term Stimulation，

Sheng-Tian Li
contact affiliation	Bio-X Institutes, Key Laboratory for the Genetics of Development and Neuropsychiatric Disorders (Ministry of Education), Shanghai Key Laboratory of Psychotic Disorders, and Brain Science and Technology Research Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
contact email	lstian@sjtu.edu.cn
lab head
Liyue Qin
contact affiliation	Kao (China) Research & Development Center
contact email	qinliyue1120@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD024018

PRIDE project URI

• PRIDE
  1. PXD024018
     1. Label: PRIDE project
     2. Name: Kininogen–Nitric Oxide Signaling at Nearby Non-excited Acupoints After Long-term Stimulation