PXD024008
PXD024008 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome from whole epididymis and caput epididymidis of WT and Ocln knockout mice |
Description | Epithelial tight junction is the frontline barrier for physiological homeostasis and protection of a host and Occludin (Ocln) is a necessary component of the tight junction complex. Male Ocln knockout (Ocln-KO) mice are overall normal but infertile. The cause of this phenotype remains elusive. We found that the infertility of Ocln-mutant males is due to defects in sperm motility and capacitation – the essential functions acquired during sperm maturation in the epididymis – but not spermatogenesis in the testis, which is normal in these mutant mice. Using epididymis as an epithelial model in Ocln-KO male mice, combining transcriptomic and proteomic analyses, we found a new unconventional phagocytosis mechanism that regulates membrane cholesterol and is featured by forming double-membrane phagosome derived from the plasma membranes of epithelial TJs and. This process, which we term paracellular heterophagy or paraphagy, involves the interaction of Ocln at the TJs with the participation of autophagy machinery and a surface receptor for lipophilic cargo endocytosis to promote cholesterol removal from TJ plasma membranes. We also provide evidence that this is a general mechanism applicable to other epithelia, including airway bronchia, renal tubules, digestive intestine and circulation endothelium, but is particularly profound in epididymis in reproductive tract. Thus, we show that paraphagy at TJs by involving Ocln is a novel mechanism which senses the luminal lipophilic signals and modulates cholesterol homeostasis in epithelia. |
HostingRepository | iProX |
AnnounceDate | 2021-02-05 |
AnnouncementXML | Submission_2024-02-03_19:17:52.755.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Baoli Zhang |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Q Exactive HF-X; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-02-05 00:23:50 | ID requested | |
⏵ 1 | 2024-02-03 19:17:53 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mice, epididymis,TMT labeled proteomics, DC2 cell lines, IP-MS/MS |
Contact List
Winnie Shum | |
---|---|
contact affiliation | ShanghaiTech University; CAS Center for excellence in Molecular Cell Science |
contact email | shumw@shanghaitech.edu.cn |
lab head | |
Baoli Zhang | |
contact affiliation | ShanghaiTech University; CAS Center for excellence in Molecular Cell Science; University of Chinese Academy of Sciences |
contact email | zhangbl@shanghaitech.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |