PXD024008

PXD024008 is an original dataset announced via ProteomeXchange.

Title	Proteome from whole epididymis and caput epididymidis of WT and Ocln knockout mice
Description	Epithelial tight junction is the frontline barrier for physiological homeostasis and protection of a host and Occludin (Ocln) is a necessary component of the tight junction complex. Male Ocln knockout (Ocln-KO) mice are overall normal but infertile. The cause of this phenotype remains elusive. We found that the infertility of Ocln-mutant males is due to defects in sperm motility and capacitation – the essential functions acquired during sperm maturation in the epididymis – but not spermatogenesis in the testis, which is normal in these mutant mice. Using epididymis as an epithelial model in Ocln-KO male mice, combining transcriptomic and proteomic analyses, we found a new unconventional phagocytosis mechanism that regulates membrane cholesterol and is featured by forming double-membrane phagosome derived from the plasma membranes of epithelial TJs and. This process, which we term paracellular heterophagy or paraphagy, involves the interaction of Ocln at the TJs with the participation of autophagy machinery and a surface receptor for lipophilic cargo endocytosis to promote cholesterol removal from TJ plasma membranes. We also provide evidence that this is a general mechanism applicable to other epithelia, including airway bronchia, renal tubules, digestive intestine and circulation endothelium, but is particularly profound in epididymis in reproductive tract. Thus, we show that paraphagy at TJs by involving Ocln is a novel mechanism which senses the luminal lipophilic signals and modulates cholesterol homeostasis in epithelia.
HostingRepository	iProX
AnnounceDate	2021-02-05
AnnouncementXML	Submission_2024-02-03_19:17:52.755.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baoli Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive HF-X; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-05 00:23:50	ID requested
⏵ 1	2024-02-03 19:17:53	announced

Dataset with its publication pending

submitter keyword: mice, epididymis,TMT labeled proteomics, DC2 cell lines, IP-MS/MS

Winnie Shum
contact affiliation	ShanghaiTech University; CAS Center for excellence in Molecular Cell Science
contact email	shumw@shanghaitech.edu.cn
lab head
Baoli Zhang
contact affiliation	ShanghaiTech University; CAS Center for excellence in Molecular Cell Science; University of Chinese Academy of Sciences
contact email	zhangbl@shanghaitech.edu.cn
dataset submitter

iProX dataset URI