PXD024005 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome Differences between Daptomycin-Resistant and WT MRSA Biofilms after Elasnin Treatment |
Description | A recent study reported that daptomycin-resistant MRSA (DAPR) strain biofilm is more resistant to daptomycin and vancomycin, as compared to the WT strain biofilm. This pose a great danger since DAPR MRSA is prevalent in clinics and they often form biofilms in medical devices. In this study, we investigate the anti-biofilm activity of elasnin against DAPR MRSA biofilms, and we observed that elasnin is not only effective to eradicate the biofilm of the DAPR strain, but it shows a superior activity compared to the susceptible WT strain. Using proteomics, we compared the proteome profile of the DAPR and the WT strain biofilm cells under elasnin treatment to reveal why elasin is superior against the DAPR strain. Besides, we also employed adaptive laboratory evolution (ALE) experiment by repetitively treating MRSA culture with high dose of elasnin, and generated evolved MRSA strain with increased elasnin tolerance. Using quantitative proteomics, we compared the proteome differences in the elasnin-susceptible WT MRSA and elasnin-tolerant evolved strain. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-11 |
AnnouncementXML | Submission_2022-03-02_02:56:30.776.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jordy Evan Sulaiman |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-04 23:14:55 | ID requested | |
1 | 2021-11-11 01:05:52 | announced | |
2 | 2022-03-02 02:56:16 | announced | 2022-03-02: Updated publication reference for PubMed record(s): 35196789. |
⏵ 3 | 2022-03-02 02:56:31 | announced | 2022-03-02: Updated publication reference for PubMed record(s): 35196789.
2022-03-02: Updated publication reference for PubMed record(s): 35076266, 35196789. |
Publication List
Sulaiman JE, Long L, Qian PY, Lam H, Elasnin Effectively Eradicates Daptomycin-Resistant Methicillin-Resistant Staphylococcus aureus Biofilms. Microbiol Spectr, 10(1):e0232021(2022) [pubmed] |
Sulaiman JE, Long L, Qian PY, Lam H, Proteomics and Transcriptomics Uncover Key Processes for Elasnin Tolerance in Methicillin-Resistant Staphylococcus aureus. mSystems, 7(1):e0139321(2022) [pubmed] |
Keyword List
submitter keyword: MRSA, elasnin, daptomycin-resistant, biofilms |
Contact List
Henry Lam |
contact affiliation | Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST) |
contact email | kehlam@ust.hk |
lab head | |
Jordy Evan Sulaiman |
contact affiliation | The Hong Kong University of Science and Technology (HKUST) |
contact email | jesulaiman@connect.ust.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024005 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024005
- Label: PRIDE project
- Name: Proteome Differences between Daptomycin-Resistant and WT MRSA Biofilms after Elasnin Treatment