PXD024003

PXD024003 is an original dataset announced via ProteomeXchange.

Title	The insufficient activation of RIG-I-like signaling pathway in IBRS-2 cells contributes to highly efficient replication of porcine picornaviruses
Description	Seneca Valley Virus (SVV) or commonly known as Senecavirus A (SVA), is one of picornavirus that is associated with vesicular disease and neonatal mortality in swine herds. However, the pathogenesis of SVV remains largely elusive. Our previous study found that SVV replicates extremely faster in porcine IBRS-2 cells than that in porcine PK-15 cells. However, the underlying mechanism remains unknown. In this study, we comprehensively compared the expression features between IBRS-2 cells and PK-15 cells in response to SVV infection by an unbiased high-throughput quantitative proteomic analysis. We found that the innate immune response-realted pathways were efficiently activated in PK-15 cells but not in IBRS-2 cells during SVV infection. A large amount of interferon-stimulated genes (ISGs) were induced in PK-15 cells. In contrast, no ISGs were induced in IBRS-2 cells after SVV infection. This different expression features in the two cell lines was verified by qPCR analysis. Besides, we determined similar results in the two cell lines during another porcine picornavirus foot-and-mouth disease virus (FMDV) infection. Further study demonstrated that the Janus kinase signal transducer and activator of transcription (JAK-STAT) signaling pathway was functioning properly in both IBRS-2 and PK-15 cells. A systematic screening study revealed that the aberrant signal transduction from TBK1 to IRF3 in the RIG-I-like receptor signaling pathway in IBRS-2 cells was the fundamental cause of the different innate immune response manifestation and different viral replication rate in the two cell lines. Together, our findings determined the different feature of IBRS-2 and PK-15 cell lines which will provide useful guidance for choosing right cell line in porcine picornaviruses-mediated host immune signaling research and could be easily extended to other porcine viruses.
HostingRepository	PRIDE
AnnounceDate	2021-10-05
AnnouncementXML	Submission_2021-10-05_02:10:07.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuying Chen
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 23:04:14	ID requested
⏵ 1	2021-10-05 02:10:08	announced

Zhang X, Yang F, Li K, Cao W, Ru Y, Chen S, Li S, Liu X, Zhu Z, Zheng H, The Insufficient Activation of RIG-I-Like Signaling Pathway Contributes to Highly Efficient Replication of Porcine Picornaviruses in IBRS-2 Cells. Mol Cell Proteomics, 20():100147(2021) [pubmed]

submitter keyword: Senecavirus A

foot-and-mouth disease virus

RIG-I like receptor signaling pathway

interferon

antiviral response

Haixue Zheng
contact affiliation	State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory,Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
contact email	haixuezheng@163.com
lab head
Shuying Chen
contact affiliation	College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi
contact email	chenshuying0709@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD024003

PRIDE project URI

• PRIDE
  1. PXD024003
     1. Label: PRIDE project
     2. Name: The insufficient activation of RIG-I-like signaling pathway in IBRS-2 cells contributes to highly efficient replication of porcine picornaviruses