PXD023996

PXD023996 is an original dataset announced via ProteomeXchange.

Title	The non-radioactive SUnSET assay to measure glucose-induced protein synthesis in human β-cells
Description	Glucose regulates the expression of a limited number of genes in human pancreatic β-cells at the transcriptional level. Here, we asked whether glucose intervenes at post-transcriptional steps and regulates protein translation in human β-cell lines. First, we showed the involvement of the m-TOR pathway in glucose-related signaling. We next used the Surface SEnsing of Translation (SUnSET) technique that is based on puromycin incorporation into newly-translated proteins to show that glucose treatment increased protein translation. Among the list of glucose-induced proteins, we selected the proconvertase PCSK1 as a promising target whose translation was induced within minutes following glucose treatment. We finally performed global proteomic analysis by mass spectrometry to characterize newly-translated proteins upon glucose treatment. We identified many proteins involved in translation, glycolysis, TCA metabolism and insulin secretion. Taken together, our study demonstrates that while glucose poorly modulates gene expression in human β-cells, it plays major role at the translational level.
HostingRepository	PRIDE
AnnounceDate	2021-07-27
AnnouncementXML	Submission_2021-07-27_06:02:19.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; mono N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 07:25:08	ID requested
⏵ 1	2021-07-27 06:02:20	announced

Dataset with its publication pending

submitter keyword: Puromycin, glucose regulation, m-TOR pathway, SUnSET

Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université de Paris - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris 75015, France
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD023996

PRIDE project URI

• PRIDE
  1. PXD023996
     1. Label: PRIDE project
     2. Name: The non-radioactive SUnSET assay to measure glucose-induced protein synthesis in human β-cells