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PXD023985

PXD023985 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome-wide identification of Lysine Methylation on non-histone of tomato (Solanum Lycopersicum) during ripening
DescriptionPost-translational modification of proteins through methylation plays important regulatory role in biological processes. Lysine methylation on histone proteins is known to play important role in chromatin structure and function. However, non-histone protein substrates of this modification remain largely unknown. Herein, we use high resolution mass spectrometry to global screening methylated substrates and lysine- methylation sites in tomato (Solanum Lycopersicum). A total of 241 sites of lysine methylation (mono-, di-, tri-methylation) in 176 proteins with diverse biological functions and subcellular localized were identified in mix tomato with different maturity. Two putative methylation motifs were detected. KEGG pathway category enrichment analysis indicated that methylated proteins are implicated in the regulation of diverse metabolic processes, including arbon fixation in photosynthetic organisms, pentose phosphate pathway, fructose and mannose metabolism, and cysteine and methionine metabolism. Three representative proteins were selected to analyze the effect of methylated modification on protein function. In addition, quantitative RT-PCR further validated the gene expression level of some key methylated proteins during fruit ripening, which are involved in oxidation reduction process, stimulus and stress, energy metabolism, signaling transduction, fruit ripening and senescence. These data represent the first report of methylation proteomic and supply abundant resources for exploring the functions of lysine methylation in tomato and other plants.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:24:01.932.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLu Xiao
SpeciesList scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationListmethylated lysine
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-04 00:08:13ID requested
12023-03-10 19:09:17announced
22023-11-14 08:24:03announced2023-11-14: Updated project metadata.
Publication List
Xiao L, Liang H, Jiang G, Ding X, Liu X, Sun J, Jiang Y, Song L, Duan X, Proteome-wide identification of non-histone lysine methylation in tomato during fruit ripening. J Adv Res, 42():177-188(2022) [pubmed]
Keyword List
submitter keyword: Lysine
Methylation
Post-translational modification
Tomato
Contact List
Lu Xiao
contact affiliationSouth China Botentical Garden, Chinese Academy of sciences
contact emailxiaolu@scbg.cac.cn
lab head
Lu Xiao
contact affiliationsouth china botentical garden,Chinese Academy of Sciences
contact emailxiaolu@scbg.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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