PXD023975

PXD023975 is an original dataset announced via ProteomeXchange.

Title	Proteome remodeling in the Mycobacterium tuberculosis PknG knockout: molecular hints for the role of this kinase in cell envelope biogenesis and hypoxia.
Description	Tuberculosis, a pulmonary disease caused by the etiological agent Mycobacterium tuberculosis, is the leading cause of death by a single infectious agent among human beings. One of M. tuberculosis's pathogenic hallmarks is its ability to persist in a dormant state in the host for long periods, reinitiating the infectious cycle when favorable environmental conditions are found. Thus, it is not surprising that this pathogen has developed different mechanisms to withstand the stressful conditions found in the host. In particular, the Ser/Thr protein kinase PknG has gained special relevance since it regulates nitrogen metabolism and facilitates bacterial survival inside macrophages. Nevertheless, the molecular mechanisms underlying these effects are far from being elucidated. To further investigate these issues, we performed quantitative proteomics analyses of protein extracts from M. tuberculosis H37Rv and a mutant derivative lacking PknG. Our results show that in the absence of PknG the mycobacterial proteome was remodeled since 6.5% of the proteins encoded by M. tuberculosis presented significant changes in its relative abundance when compared to the wild-type strain. The main biological processes affected by PknG deletion were the biosynthesis of cell envelope components and the response to hypoxic conditions. Altogether, the results presented here allow us to postulate that PknG regulation of bacterial fitness to stress conditions in host macrophages might be an essential mechanism underlying its reported effect on intracellular bacterial survival.
HostingRepository	PRIDE
AnnounceDate	2021-06-02
AnnouncementXML	Submission_2021-06-01_22:28:47.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Analía Lima
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-03 09:01:12	ID requested
⏵ 1	2021-06-01 22:28:48	announced

Lima A, Leyva A, Rivera B, Portela MM, Gil M, Cascioferro A, Lisa MN, Wehenkel A, Bellinzoni M, Carvalho PC, Batthy, á, ny C, Alvarez MN, Brosch R, Alzari PM, Dur, á, n R, Proteome remodeling in the Mycobacterium tuberculosis PknG knockout: Molecular evidence for the role of this kinase in cell envelope biogenesis and hypoxia response. J Proteomics, 244():104276(2021) [pubmed]

submitter keyword: Mycobacterium tuberculosis, Serine/Threonine protein kinase, PknG, Mas, Msl3, hypoxia, Hrp-1, shotgun proteomics, parallel reaction monitoring

Rosario Durán
contact affiliation	Institut Pasteur de Montevideo & Insituto de Investigaciones Biológicas Clemente Estable, Analytical Biochemistry and Proteomics Unit, Montevideo, Uruguay
contact email	duran@pasteur.edu.uy
lab head
Analía Lima
contact affiliation	Institut Pasteur de Montevideo, Proteomic and Analytical Biochemistry Unit
contact email	alima@pasteur.edu.uy
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD023975

PRIDE project URI

• PRIDE
  1. PXD023975
     1. Label: PRIDE project
     2. Name: Proteome remodeling in the Mycobacterium tuberculosis PknG knockout: molecular hints for the role of this kinase in cell envelope biogenesis and hypoxia.