PXD023973

PXD023973 is an original dataset announced via ProteomeXchange.

Title	Label-free shotgun proteomics of tmRNA substrates enriched by immunoprecipitation
Description	We used a combination of genetic and proteomic approaches to characterize tmRNA (ssrA) activity in the genome-reduced bacterium Mycoplasma pneumoniae. For this, we generated tmRNA mutants encoding a tag resistant to proteolysis. Endogenous protein tagging by the mutant tmRNA gene (ssrAmk) was then examined by immunoprecipitation (IP) enrichment followed by LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2021-08-23
AnnouncementXML	Submission_2021-08-23_06:49:54.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Weber
SpeciesList	scientific name: Mycoplasma pneumoniae M129; NCBI TaxID: 272634;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-03 09:00:21	ID requested
⏵ 1	2021-08-23 06:49:55	announced

Dataset with its publication pending

submitter keyword: Mycoplasma pneumoniae, tmRNA, trans-translation, protein degradation, ribosome stalling

Luis Serrano
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain
contact email	luis.serrano@crg.eu
lab head
Marc Weber
contact affiliation	Centre for Genomic Regulation
contact email	marc.weber@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD023973

PRIDE project URI

• PRIDE
  1. PXD023973
     1. Label: PRIDE project
     2. Name: Label-free shotgun proteomics of tmRNA substrates enriched by immunoprecipitation