PXD023952

PXD023952 is an original dataset announced via ProteomeXchange.

Title	Metabolic flexibility and expression of CD98 define the low density neutrophils mobilised in response to G-CSF
Description	Low density neutrophils (LDN) co-purify with mononuclear cells during density-gradient separation of peripheral blood. LDN are rarely detected in healthy individuals but appear in the peripheral blood (PB) of patients with inflammatory diseases, malignancies and in individuals treated with granulocyte colony-stimulating factor (G-CSF). Using human G-CSF mobilized LDN, this study reveals that LDN are transcriptionally, proteomically and metabolically distinct from other neutrophils. Single Cell RNAseq analysis reveals that LDN from these donors comprise one major population, with multiple interrelated transcriptional states, representing the continuum of neutrophil maturation. LDN can engage both glycolysis and mitochondrial respiration to meet energy demands, which affords increased metabolically flexibility in the nutrient perturbed tissue environments found in tumours and inflammatory diseases. This study also identifies upregulated expression of CD98, a transporter for branch chain and aromatic amino acids, as a cell surface marker for LDN in peripheral blood. CD98 may contribute to the functional properties of LDNs and represents a useful phenotypic marker.
HostingRepository	PRIDE
AnnounceDate	2022-12-19
AnnouncementXML	Submission_2022-12-19_08:42:14.602.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JarrodSandow
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-02 23:27:17	ID requested
⏵ 1	2022-12-19 08:42:14	announced

Dataset with its publication pending

submitter keyword: proteogenomics, G-CSF,neutrophils

IanWicks
contact affiliation	Laboratory Head
contact email	wicks@wehi.edu.au
lab head
JarrodSandow
contact affiliation	WEHI
contact email	sandow@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD023952

PRIDE project URI

• PRIDE
  1. PXD023952
     1. Label: PRIDE project
     2. Name: Metabolic flexibility and expression of CD98 define the low density neutrophils mobilised in response to G-CSF