PXD023940

PXD023940 is an original dataset announced via ProteomeXchange.

Title	Borrelia burgdorferi infected Ixodes scapularis nymph saliva proteome
Description	Lyme disease (LD) is one of the most important human vector-borne diseases in North America. Since transmission of Borrelia burgdorferi (Bb), the causative agent of LD, is influenced by yet unknown tick saliva proteins (TSP), the discovery and characterization of such TSPs are highly sought after for their potential as tick-antigen based vaccine targets. We validated a novel non-invasive approach of collecting sufficient amounts of saliva from unfed, partially fed (12, 24, 36, 48, 60, and 72h), and replete fed Ixodes scapularis nymphs for identification of tick saliva proteins using the LC-MS/MS approach. Our data has described TSPs that might be injected into the host within few hours of the tick starting to feed and are likely associated with manipulating host immunity to facilitate transmitted Bb to colonize the host. Thus, these data will serve as a source for Bb-infection associated TSPs that might be targeted in tick-antigen based vaccines to prevent LD. Equally important, the non-invasive method to collect nymph tick saliva is likely to provide transformative impact on research to understand transmission of other TBD agents.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-10_00:47:27.838.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023940
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Albert Mulenga
SpeciesList	scientific name: Borreliella burgdorferi; NCBI TaxID: 139; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986; scientific name: Ixodes scapularis; NCBI TaxID: 6945;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-02 01:58:46	ID requested
⏵ 1	2021-09-10 00:47:28	announced

Kim TK, Tirloni L, Bencosme-Cuevas E, Kim TH, Diedrich JK, Yates JR, Mulenga A, Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs. BMC Genomics, 22(1):152(2021) [pubmed]

submitter keyword: tick saliva, Borrelia burgdorferi, rabbit host

Albert Mulenga
contact affiliation	Texas A&M University College of Biomedical Sciences and Veterinary Medicine Department of Veterinary Pathobiology Mulenga Laboratory College Station, Texas, USA 77843
contact email	amulenga@cvm.tamu.edu
lab head
Albert Mulenga
contact affiliation	Texas A&M University
contact email	mulengalab06@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023940

PRIDE project URI

• PRIDE
  1. PXD023940
     1. Label: PRIDE project
     2. Name: Borrelia burgdorferi infected Ixodes scapularis nymph saliva proteome