PXD023931

PXD023931 is an original dataset announced via ProteomeXchange.

Title	Identification of GmMYB176 interactome in soybean by Co-immunoprecipitation and LC-MS/MS
Description	GmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the isoflavonoid biosynthetic pathway thereby affecting isoflavonoid level in soybean roots. While GmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function and requires additional cofactor (s). The aim of this study was to identify GmMYB176 interactome for the regulation of isoflavonoid biosynthesis in soybean. GmMYB176 interactome was identified by Co-Immunoprecipitation using two YFP-tagged proteins baits, N’-GmMYB176-YFP-C’ and N’-YFP-GmMYB176-C’. GmMYB176 interacting proteins were identified by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2021-02-03
AnnouncementXML	Submission_2021-02-02_18:15:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arun Kumaran Anguraj Vadivel
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-02 01:42:31	ID requested
⏵ 1	2021-02-02 18:15:29	announced

Dataset with its publication pending

submitter keyword: Soybean, GmMYB176, Co-IP, Gel-based proteomics, LC-MS, PPI, Proteomics, Soybean

Sangeeta Dhaubhadel
contact affiliation	London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON Canada
contact email	sangeeta.dhaubhadel@canada.ca
lab head
Arun Kumaran Anguraj Vadivel
contact affiliation	London Research and Development Centre
contact email	arunkumaran07.ak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023931

PRIDE project URI

• PRIDE
  1. PXD023931
     1. Label: PRIDE project
     2. Name: Identification of GmMYB176 interactome in soybean by Co-immunoprecipitation and LC-MS/MS