PXD023928
PXD023928 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of pomalidomide-dependent CRBN binding proteins |
Description | The CRL4-FH-CRBN complex was purified from 293T cells stably expressing FH-CRBN and stained with CBB following SDS-PAGE. The purified complex was incubated with various cell extracts for 24 h in the presence or absence of pomalidomide. Bound proteins were then affinity-purified using anti-HA beads and subjected to SDS PAGE and CBB staining. Gel slices were prepared and subjected to MS. |
HostingRepository | jPOST |
AnnounceDate | 2022-02-02 |
AnnouncementXML | Submission_2022-09-18_03:10:23.786.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Takao Kawakami |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-02-02 00:16:51 | ID requested | |
1 | 2022-02-01 07:00:03 | announced | |
⏵ 2 | 2022-09-18 03:10:24 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: CRBN, pomalidomide, PLZF (ZBTB16) |
Contact List
Takumi Ito and Hiroshi Handa | |
---|---|
lab head | |
Takao Kawakami | |
contact affiliation | Medical ProteoScope Co. |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001075/ |