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PXD023928

PXD023928 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of pomalidomide-dependent CRBN binding proteins
DescriptionThe CRL4-FH-CRBN complex was purified from 293T cells stably expressing FH-CRBN and stained with CBB following SDS-PAGE. The purified complex was incubated with various cell extracts for 24 h in the presence or absence of pomalidomide. Bound proteins were then affinity-purified using anti-HA beads and subjected to SDS PAGE and CBB staining. Gel slices were prepared and subjected to MS.
HostingRepositoryjPOST
AnnounceDate2022-02-02
AnnouncementXMLSubmission_2022-09-18_03:10:23.786.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTakao Kawakami
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-02 00:16:51ID requested
12022-02-01 07:00:03announced
22022-09-18 03:10:24announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CRBN, pomalidomide, PLZF (ZBTB16)
Contact List
Takumi Ito and Hiroshi Handa
lab head
Takao Kawakami
contact affiliationMedical ProteoScope Co.
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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