PXD023920 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Triple SILAC identified progesterone-independent and dependent PRA and PRB interacting partners in human breast cancer cells |
Description | Progesterone receptor (PR) isoforms, PRA and PRB, both progesterone-independent and dependent modulated the biology of breast cancer cells. The different phenotypes in breast cancer mediated by PRA and PRB could due to the differences of their structures, leading to the distinct protein interacting partners and downstream signaling events of each receptor. Here, we constructed Tet-inducible HA-tagged PRA or HA-tagged PRB in T47DC42 breast cancer cells. We performed affinity purification coupled with SILAC mass spectrometry technique to comprehensively study PRA and PRB interacting partners in both liganded and unliganded conditions. To validate our findings, we applied both forward and reverse SILAC to effectively minimize experimental errors. These datasets will be useful in investigating PRA- and PRB-specific molecular mechanisms and can potentially be used as a database for subsequent experiments to identify novel PRA and PRB interacting proteins that differentially mediated different biological functions in breast cancer cells. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-08 |
AnnouncementXML | Submission_2021-03-08_06:36:41.825.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Prangwan Pateetin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-01 06:49:44 | ID requested | |
⏵ 1 | 2021-03-08 06:36:42 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Breast cancer, Progesterone receptor, SILAC, Interacting partners |
Contact List
Viroj Boonyaratanakornkit |
contact affiliation | Age-related Inflammation and Degeneration Research Unit, Chulalongkorn University, Bangkok 10330, Thailand |
contact email | Viroj.b@chula.ac.th |
lab head | |
Prangwan Pateetin |
contact affiliation | Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand |
contact email | prangwan.pa@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023920
- Label: PRIDE project
- Name: Triple SILAC identified progesterone-independent and dependent PRA and PRB interacting partners in human breast cancer cells