PXD023920

PXD023920 is an original dataset announced via ProteomeXchange.

Title	Triple SILAC identified progesterone-independent and dependent PRA and PRB interacting partners in human breast cancer cells
Description	Progesterone receptor (PR) isoforms, PRA and PRB, both progesterone-independent and dependent modulated the biology of breast cancer cells. The different phenotypes in breast cancer mediated by PRA and PRB could due to the differences of their structures, leading to the distinct protein interacting partners and downstream signaling events of each receptor. Here, we constructed Tet-inducible HA-tagged PRA or HA-tagged PRB in T47DC42 breast cancer cells. We performed affinity purification coupled with SILAC mass spectrometry technique to comprehensively study PRA and PRB interacting partners in both liganded and unliganded conditions. To validate our findings, we applied both forward and reverse SILAC to effectively minimize experimental errors. These datasets will be useful in investigating PRA- and PRB-specific molecular mechanisms and can potentially be used as a database for subsequent experiments to identify novel PRA and PRB interacting proteins that differentially mediated different biological functions in breast cancer cells.
HostingRepository	PRIDE
AnnounceDate	2021-03-08
AnnouncementXML	Submission_2021-03-08_06:36:41.825.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prangwan Pateetin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-01 06:49:44	ID requested
⏵ 1	2021-03-08 06:36:42	announced

Dataset with its publication pending

submitter keyword: Breast cancer, Progesterone receptor, SILAC, Interacting partners

Viroj Boonyaratanakornkit
contact affiliation	Age-related Inflammation and Degeneration Research Unit, Chulalongkorn University, Bangkok 10330, Thailand
contact email	Viroj.b@chula.ac.th
lab head
Prangwan Pateetin
contact affiliation	Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
contact email	prangwan.pa@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023920
     1. Label: PRIDE project
     2. Name: Triple SILAC identified progesterone-independent and dependent PRA and PRB interacting partners in human breast cancer cells