PXD023918
PXD023918 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BioID mass spectrometry analysis of proteins proximal to human NERCLIN |
| Description | To gain insight into the function of NERCLIN in human cells, we analyzed its proximal proteins by the proximity-dependent biotin identification (BioID) approach. 143B cells were transfected with fusion constructs using jetPRIME. Following 24 h of transfection, biotin was added to each plate and allowed to biotinylate the proximal proteins for the next 24 h. The plates were washed with PBS, cells were scraped, pelleted and frozen. For each sample, four biological replicates were analyzed. Liquid chromatography mass spectrometry analysis was performed on an Orbitrap Elite ETD mass spectrometer (Thermo Scientific) using the Xcalibur version 2.7.1 coupled to a Thermo Scientific nLCII nanoflow system. The mass spec analysis was done in technical duplicates. Thermo.RAW files were searched with Proteome Discoverer 1.4 (Thermo Scientific) against SEQUEST search engine. The search parameters were taken from. All reported data were based on high confidence peptides assigned in Proteome Discoverer with a 0.01% FDR by Percolator. Control samples (GFP and AIF) and Contaminant Repository for Affinity Purification database were used to identify high confidence interactors of NERCLIN based on the spectral counts. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-03-08 |
| AnnouncementXML | Submission_2022-03-08_00:29:32.972.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Svetlana Konovalova |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Biotin |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-02-01 03:27:30 | ID requested | |
| ⏵ 1 | 2022-03-08 00:29:33 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Human, 143B cells, NERCLIN, mitochondria |
Contact List
| Markku Varjosalo | |
|---|---|
| contact affiliation | University of Helsinki |
| contact email | markku.varjosalo@helsinki.fi |
| lab head | |
| Svetlana Konovalova | |
| contact affiliation | University of Helsinki |
| contact email | svetlana.konovalova@helsinki.fi |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086786/ |
to receive all new ProteomeXchange dataset release announcements!
